TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Genome editing in the unicellular holozoan Capsaspora owczarzaki suggests a premetazoan role for the Hippo pathway in multicellular morphogenesis.
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coYki is dispensable for cell proliferation in Capsaspora.
"we observed no significant difference in cell proliferation between WT and coYki -/- cells in either condition"
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coYki -/- aggregates are flatter and less circular but of normal size.
"Computational analysis of aggregate morphology and size revealed that WT and coYki -/- aggregate size was not significantly different"
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coYki -/- cells form excessive actin-depleted blebs. The phenotype is rescued by a coYki transgene and suppressed by blebbistatin.
"These results suggest that the protrusions observed in coYki -/- cells are actin-depleted blebs"
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Tagged coYki is cytoplasm-enriched in wild-type cells. Mutating its HXRXXS motifs (4SA) shifts it into the nucleus.
"These results show that HXRXXS motifs affect the subcellular localization of coYki and suggest that, like in animals, phosphorylation of coYki by Hippo signaling leads to cytoplasmic sequestration."
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coYki loss alters the expression of 1205 genes, with actin-binding genes enriched among up-regulated genes and no proliferation or cell-cycle enrichment.
"no enrichment was detected in functional categories of cell proliferation or the cell cycle"
The Hippo kinase cascade regulates a contractile cell behavior and cell density in a close unicellular relative of animals.
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Loss of coHpo or coWts increases nuclear localization of coYki.
"Together, these results show that the Capsaspora Hippo kinase cascade regulates coYki by cytoplasmic sequestration"
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Hyperactive coYki 4SA phenocopies the kinase mutants (increased aggregate cell packing, elongated cells).
"stable transgenic expression of coYki 4SA resulted in increased cell packing of Capsaspora aggregates"
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The 4SA phenotypes require the TEAD-interface residue (F123A abolishes them).
"This result indicates that these phenotypes are mediated by the transcriptional activity of coYki."
Premetazoan origin of the hippo signaling pathway.
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Co-Yki alone does not drive fly eye overgrowth, but Co-Yki plus Co-Sd does.
"co-expression of Co-Yki and Co-Sd (GMR>Co-Sd+Co-Yki) resulted in massive tissue overgrowth resembling that caused by co-expression of their Drosophila counterparts"
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Co-Yki and Co-Sd co-immunoprecipitate and activate an HRE reporter.
"epitope-tagged Co-Sd and Co-Yki immunoprecipitated with each other in Drosophila S2R+ cells"
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Co-Hpo induces Co-Yki phosphorylation and inhibits Co-Sd/Co-Yki activity.
"Co-Hpo significantly inhibited Co-Sd/Co-Yki-mediated activation of the diap1 HRE-luciferase reporter in S2R+ cells"
Assembling a Hippo: the evolutionary emergence of an animal developmental signaling pathway.
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coYki has four putative Warts phosphorylation sites, three of them conserved with Yorkie/YAP/TAZ.
"coYki also contains four putative Warts phosphorylation sites"
Regulated aggregative multicellularity in a close unicellular relative of metazoa.
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Capsaspora has a regulated aggregative multicellular stage.
"we describe an aggregative multicellular stage in the protist Capsaspora owczarzaki, a close unicellular relative of metazoans"