Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
An adenylyl cyclase that functions during late development of Dictyostelium.
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acrA encodes a novel adenylyl cyclase expressed at low levels in growth and induced more than 25-fold during development.
"we discovered a novel adenylyl cyclase gene, acrA, that is expressed at low levels in growing cells and at more than 25-fold higher levels during development"
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acrA null cells develop normally to the slug stage but fail to make viable spores and produce unnaturally long stalks.
"Growth and development up to the slug stage are unaffected in acrA(-) mutant strains but the cells make almost no viable spores and produce unnaturally long stalks"
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ACR is dispensable until culmination when it is critical for sporulation and stalk construction.
"As long as ACA is fully active, ACR is not required until culmination but then plays a critical role in sporulation and construction of the stalk"
The genome of the social amoeba Dictyostelium discoideum.
Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG.
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Caffeine inhibits cAMP accumulation by ACB (acrA) as well as ACA and ACG in intact cells.
"Caffeine, which was previously used to specifically block ACA function, also inhibited cAMP accumulation by ACB and ACG"
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ACB, encoded by the AcrA gene, is required for spore maturation.
"ACB is required for the maturation of spores"
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
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acrA mutants show phenotypes only at later (slug-to-culmination) developmental stages, consistent with a late-development role.
"In contrast, dhkA and acrA show mutant phenotypes only at later stages consistent with their specific roles during slug to culmination stage"
Cytokinins induce sporulation in Dictyostelium.
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AcrA and the histidine kinase DhkB are both required to transduce the cytokinin (discadenine) signal that triggers spore encapsulation.
"DhkB and the adenylyl cyclase of late development, AcrA, are members of two component signal transduction families and both are required to transduce the cytokinin signal"
Functional dissection of adenylate cyclase R, an inducer of spore encapsulation.
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ACR is essential for spore encapsulation and its multidomain architecture includes seven TM helices, a histidine kinase domain, and two receiver domains.
"In addition to its cyclase (AC) domain, ACR harbors seven transmembrane helices, a histidine kinase domain, and two receiver domains"
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ACR localizes to the endoplasmic reticulum and nuclear envelope.
"This strongly suggests that ACR localizes to the endoplasmic reticulum and nuclear envelope"
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ACR activity is not critically regulated by phosphorelay, and its intramolecular histidine kinase activity is dispensable.
"indicating that AC activity is not critically regulated by phosphorelay"
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The degenerate HisKA subdomain lacks the canonical autophosphorylation histidine yet is essential for AC activity, promoting dimerization of the cyclase domain.
"the dimerizing histidine phosphoacceptor subdomain, which in ACR lacks the canonical histidine for autophosphorylation, was essential for AC activity"
Transcriptomic and metabolomic insights into light-mediated unicellular-to-multicellular transition in Dictyostelium discoideum.
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acrA is a light-responsive differentially expressed gene, downregulated in the dark in the unicellular phase.
"Here we also observed that the adenylate cyclase gene acrA was downregulated under dark in the unicellular phase"