Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Peptide signaling during terminal differentiation of Dictyostelium.
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The peptide sporulation signal SDF-2 is generated by proteolytic processing of the acyl-CoA-binding protein AcbA.
"the peptide signal spore differentiation factor 2 (SDF-2) is processed from acyl-CoA binding protein, AcbA"
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Recombinant AcbA binds palmitoyl-CoA in a 1:1 molar ratio.
"AcbA was able to bind C 14 -palmitoyl-CoA in a 1:1 molar ratio"
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AcbA-null mutants fail to make SDF-2 and are rescued in chimeras with wild-type cells, indicating a non-cell-autonomous intercellular signal.
"Mutants lacking AcbA sporulate well only when developed in chimeras with WT cells"
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SDF-2 acts as a ligand for the histidine kinase DhkA, inhibiting its phosphorelay to induce rapid sporulation.
"ligand binding to the SDF-2 receptor histidine kinase, DhkA, inhibits phosphorelay, which can account for its ability to induce rapid sporulation"
Global transcriptional responses to cisplatin in Dictyostelium discoideum identify potential drug targets.
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An acbA mutant shows altered cisplatin sensitivity, linking AcbA to sphingolipid metabolism-dependent drug response rather than to a direct DNA damage response.
"Mutations in five cisplatin response genes (sgkB, csbA, acbA, smlA, and atg8) resulted in altered drug sensitivity"
Unconventional secretion of AcbA in Dictyostelium discoideum through a vesicular intermediate.
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AcbA is unconventionally secreted and processed into SDF-2.
"The acyl coenzyme A (CoA) binding protein AcbA is secreted unconventionally and processed into spore differentiation factor 2 (SDF-2)"
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AcbA localizes to cortical membrane-bound vesicles in prespore cells that act as secretion intermediates.
"AcbA is localized in vesicles that accumulate in the cortex of prespore cells just prior to sporulation"
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Extracellular AcbA is cleaved by the prestalk surface protease TagC during culmination.
"During culmination, extracellular AcbA is rapidly cleaved by the TagC protease that is exposed on the surface of prestalk cells"
Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.
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acbA expression dynamics fit a vegetative housekeeping role plus a late-development spore-differentiation signaling role.
"The expression profile of acbA was consistent with its known housekeeping function during vegetative growth, and a signaling function in terminal spore differentiation"