Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Overexpression of the Notch target genes Hes in vivo induces lymphoid and myeloid alterations.
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HES1 represses E2A (E-protein) transcriptional activity in reporter assays and impairs B-cell differentiation, demonstrating its repressor function and antagonism of proneural/E-protein bHLH activators.
"their ability to interfere with the transcriptional activity of E2A in a reporter assay was comparable to that of Notch1IC"
Human Sir2-related protein SIRT1 associates with the bHLH repressors HES1 and HEY2 and is involved in HES1- and HEY2-mediated transcriptional repression.
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Human HES1 physically associates with the deacetylase SIRT1, and both SIRT1-dependent and -independent deacetylase pathways contribute to HES1-mediated transcriptional repression.
"SIRT1, also physically associates with the human bHLH repressor proteins, hHES1 and hHEY2, both in vitro and in vivo"
Conservation of the Notch1 signaling pathway in gastrointestinal carcinoid cells.
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Inducible Notch1 activation in human carcinoid cells increases HES1 protein, confirming HES1 as a downstream effector of Notch signaling.
"Notch1 pathway activation led to an increase in hairy enhancer of split 1 (HES-1) protein"
Notch signaling is required for maintaining stem-cell features of neuroprogenitor cells derived from human embryonic stem cells.
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HES1 is expressed downstream of Notch in human hESC-derived neuroprogenitors; Notch inhibition reduces NSC markers and proliferation and triggers neuronal differentiation, implicating HES1 in neural stem-cell maintenance.
"Inhibition of the Notch signaling by a gamma-secretase inhibitor reduced rosette structures, expression levels of NSC marker genes and proliferation potential"
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
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Human HES1 DNA-binding specificity was determined directly by methylation-sensitive SELEX in a genome-scale transcription-factor study, supporting sequence-specific double-stranded DNA binding.
"systematic analysis of DNA binding specificities of full-length TFs and eDBDs using unmethylated and CpG-methylated DNA ligands"
Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements.
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HES-1 binds N-box (CACNAG) elements preferentially over E-box, acts as a negative regulator, and negatively autoregulates its own promoter through N-box sequences.
"it binds more preferentially to the N box (CACNAG) than to the E box (CANNTG) and acts as a negative regulator"
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HES-1 represses promoter activity ~40-fold, and disrupting the N-box sequences severely impairs this autoregulation.
"cotransfection of the HES-1 expression vector leads to approximately 40-fold repression in promoter activity"
Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy.
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The human hairy homolog (HRY/HES1) is structurally homologous to Drosophila hairy and the rat RHL immediate-early gene that suppresses neuronal differentiation, linking HES1 to nervous system development.
"can, like that in Drosophila, suppress neuronal differentiation events"
NOTCH1 PEST domain mutants stimulate HES1 transcription
HES1 gene transcription is inhibited by RUNX3
HES1 gene expression is stimulated by NOTCH4
Deep research report on HES1
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HES1 is a nuclear bHLH/Orange/WRPW transcriptional repressor and canonical Notch effector that binds N-box/class C motifs, recruits Groucho/TLE via WRPW, and oscillates to control developmental fate decisions.
"Human HES1 (Q14469) is a nuclear bHLH/Orange/WRPW transcriptional repressor and canonical NOTCH effector that binds N-box/class C motifs and recruits Groucho/TLE through WRPW to silence targets."