Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Automatic Gene Ontology annotation based on Rhea mapping
Pre-steady-state DNA unwinding by bacteriophage T4 Dda helicase reveals a monomeric molecular motor.
-
Monomeric Dda rapidly unwinds oligonucleotide duplexes in pre-steady-state assays without requiring oligomerization.
"Thus, Dda can rapidly unwind oligonucleotide substrates as a monomer, indicating that the functional molecular motor component of a helicase can reside within a single polypeptide."
Overexpression, purification, sequence analysis, and characterization of the T4 bacteriophage dda DNA helicase.
-
Dda is a 5'-3' DNA helicase and DNA-dependent ATPase that stimulates T4 DNA replication and recombination in vitro and contributes to replication initiation in vivo.
"The bacteriophage T4 dda protein is a 5'-3' DNA helicase that stimulates DNA replication and recombination reactions in vitro and seems to play a role in the initiation of T4 DNA replication in vivo."
The phage T4 protein UvsW drives Holliday junction branch migration.
-
Together with the UvsX recombinase, Dda helicase promotes template strand switching by T4 DNA polymerase.
"In this system, the strand exchange protein UvsX and the Dda helicase promoted strand switching by T4 polymerase."
A role for two DNA helicases in the replication of T4 bacteriophage DNA.
-
Dda provides an alternative helicase pathway that allows the T4 replication machinery to bypass DNA-bound RNA polymerase barriers.
"A second, very different DNA helicase, the T4 dda protein, provides an alternative pathway for replication past this DNA-bound RNA polymerase"
UniProtKB record for P32270 (DDA_BPT4)
-
Dda unwinds DNA as a monomer in a 5'-3' direction at ~250 bp/s, displaces DNA-bound proteins, and stimulates T4 DNA replication initiation and recombination.
"DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA (PubMed:17823128, PubMed:22504228)."
Deep research summary for Enterobacteria phage T4 helicase dda
-
Dda is a superfamily 1B helicase that couples ATP hydrolysis to 5'-3' translocation on ssDNA and unwinding of dsDNA junctions.
"Dda is a helicase that uses **ATP hydrolysis** to move along **single-stranded DNA (ssDNA)** and unwind **double-stranded DNA (dsDNA)** junctions encountered during translocation."