Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Mechanisms of copper loading on the Schizosaccharomyces pombe copper amine oxidase 1 expressed in Saccharomyces cerevisiae.
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Heterologously expressed Cao1 is an active primary amine oxidase.
"Expression of
spao1(+) resulted in the production of an active enzyme capable of catalysing
the oxidative deamination of primary amines."
Copper distributed by Atx1 is available to copper amine oxidase 1 in Schizosaccharomyces pombe.
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Atx1 is a major copper source for Cao1; an Atx1-independent route also exists.
"the Atx1 metallochaperone represents an important source of copper for Cao1."
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Cao1, not Cao2, accounts for amine oxidase activity in S. pombe.
"In contrast, no detectable CAO activity was observed with the cao1 Δ single mutant (Fig. 3A )."
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Functional Cao1-GFP is cytosolic and excluded from the nucleus.
"By use of direct fluorescence microscopy, Cao1-GFP exhibited uniform fluorescent staining throughout the cytosol."
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Cao1 oxidized every primary amine tested (unpublished panel); best substrates ethylamine, putrescine and 1,8-diaminooctane.
"The best substrates for Cao1 were ethylamine, putrescine, and 1,8-diaminooctane, while the aromatic monoamine benzylamine was oxidized less efficiently"
Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.
Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination.
UniProtKB Q9P7F2: curated sequence, features and source attribution