The evidence supports one main non-lysosomal glucosylceramidase role, with secondary chemistry and cellular consequences kept distinct. Direct original assays and their species/construct limits are summarized in the source guide and reference assessments (PMID:17080196(https://pubmed.ncbi.nlm.nih.gov/17080196/); PMID:26724485(https://pubmed.ncbi.nlm.nih.gov/26724485/); PMID:32144204(https://pubmed.ncbi.nlm.nih.gov/32144204/)).
ER/Golgi and plasma-membrane observations describe different experimental contexts rather than a resolved universal topology (PMID:23250757(https://pubmed.ncbi.nlm.nih.gov/23250757/); PMID:30308956(https://pubmed.ncbi.nlm.nih.gov/30308956/)). Smooth ER specificity remains unresolved. The three natural product sequences are verified; product-specific catalytic and localization differences remain open.
The manual synthesis records source sections, constructs and experimental limits behind the hydrolysis/transfer distinction and membrane-pool interpretation. GalCer analog hydrolysis remains a measured result, while its physiological contribution is unresolved; its existing annotation is now marked over-annotated and is no longer proposed as a replacement for generic hydrolase activity. Selective excerpts make the cached cytoskeletal, surface-activity and sterol-hydrolysis evidence directly checkable. The earlier notes remain intact.
The reviewed human record independently curates GalCer hydrolysis with its in-vitro lower-activity qualification, bile-acid glucosyl transfer and reported plasma-membrane localization/alternative topologies. These statements corroborate the inspected original assays without resolving physiological GalCer clearance or universal topology. Purified-human bile-acid glucoside cleavage is linked directly to PMID:9111029. The proposed glycoside-catabolism route to carbohydrate metabolism has no verified is_a/part_of path, so the carbohydrate assertion is retained on its measured chemistry. Reverse GlcCer formation and sterol-glucoside formation retain distinct product scope.