GSTF11 (Q96324, At3g03190) review notes

Session 2026-09-30

Identity and biochemistry

• Phi-class GST (PANTHER PTHR43900:SF64). UniProt function and EC are by similarity to GSTF9 (O80852).
• No direct enzymology: recombinant protein insoluble PMID:19174456; also PMID:35145536.
• Indirect in vivo support: overexpression in Brassica napus raises leaf GST activity PMID:34961200.

Glucosinolates

• MYB28-regulated PMID:17420480.
• CRISPR gstf11: some aliphatic GSLs reduced, indolic unaffected PMID:35145536; weaker than gstu20; authors note the gstf11 lesions are late in the gene (possible partial function).
• Mikkelsen et al. 2010 glucoraphanin engineering in tobacco (PMID:20457641, abstract-only) is cited by Zhang et al. as showing GSTF11 is not essential for heterologous production; not verified from the abstract.
• Decision: no NEW GO:0019761, same reasoning as GSTU20 (see GSTU20-notes.md): necessity evidence only, no activity on the native intermediate, non-null double mutant, possible non-enzymatic conjugation. Comparators: GSTF9/GSTF10 lack glucosinolate terms; GSTU13 has IMP indole glucosinolate catabolic process (acts_upstream_of_or_within).

Stress

• B. napus 35S:AtGSTF11 lines: reduced powdery mildew growth PMID:34961200; transgene cold-responsive. Heterologous and correlative; no NEW defense/cold terms proposed.
• IEP response to oxidative stress (PMID:9449849): Al-induced GST clone identified only by identity to a Kiyosue et al. 1993 GST; locus not named and oxidative-stress link is by citation -> UNDECIDED.

Action tally

• ACCEPT 7, KEEP_AS_NON_CORE 3, UNDECIDED 1, NEW 0 (11 rows).

2026-10-15 journal: response to oxidative stress row resolved (PR #5026 follow-up)

Supersedes the "IEP response to oxidative stress (PMID:9449849) ... -> UNDECIDED" line and the action tally above (now ACCEPT 7, KEEP_AS_NON_CORE 3, REMOVE 1).

• GO:0006979 IEP (PMID:9449849) is REMOVE. The cached full text identifies the Al-induced clone only by >98% identity to the "GST ( Kiyosue et al., 1993 )", in the singular, without saying which Kiyosue clone. Kiyosue et al. 1993 (PMID:8253194) described two cDNAs, ERD11 and ERD13.
• Clone identities (UniProt): ERD11 = GSTF6 (P42760, At1g02930; GSTF6-uniprot.txt Synonyms=ERD11, GST1, GSTF3); ERD13 = GSTF10 (P42761, At2g30870; downloaded 2026-10-15 from https://rest.uniprot.org/uniprotkb/P42761.txt, Synonyms=ERD13). GSTF11 is At3g03190 (Q96324), legacy alias AtGSTF6.
• Sequence check (GSTF11-bioinformatics/pairwise_identity.py, output in RESULTS.md): global identity GSTF11 vs GSTF6/ERD11 39.6% (over alignment), GSTF11 vs GSTF10/ERD13 42.7%. Neither is compatible with a >98%-identical clone, so the clone is GSTF6 or GSTF10, not GSTF11. (An earlier "~42%" figure in the review reason was not recorded; it is replaced by these reproducible numbers.)
• The oxidative-stress link in the paper is by citation of ozone work, not measured for this clone.

Module-member evidence audit — 2026-10-25

Reviewed every original row (11) and core function using review/annotation-reviewer instructions, current cached primary publications and the existing research. Preserved all source annotation fields and all pre-existing proposed rows. Reused the existing genuine Falcon provider report and its artifacts. These plant targets do not require bacterial FEBA retrieval.

Full Methods of PMID34961200 identifies AtGSTF11 At3g03190/NM_111189.3 amplified from Arabidopsis cDNA and introduced into B.napus. Increased total extract GST activity is supportive of the actual target transgene, but not purified target kinetics or native-conjugate chemistry. PMID35145536 provides Arabidopsis genetics and does not establish the isolated native reaction. Re-read the existing reproducible identity report and PMID9449849 attribution argument; retained its source-specific oxidative-stress removal, not a general denial of all stress roles. Toxin catabolism remains unresolved.

Broad and substrate-specific terms were assessed consistently across evidence codes. No NEW rows were added. Previously proposed rows were evaluated for direct participation rather than filled from pathway adjacency. Experimental cohort calls whose protein-level data were not verified were left UNDECIDED; full-text availability alone was not treated as proof of a target-specific assay. Original sources and provider reports were not hand-edited.

Evidence-field cleanup, 2026-10-26

Kept the alias crosswalk in the rationale and removed protein/gene-name quotes from supporting evidence. The experimental citation and reproducible sequence-comparison findings independently support the source attribution assessment.

2026-10-26 — PR #5550 source-level correction

Revisited review findings 1–3 against the actual publication cache and the primary sources. Removed generic source-unresolved boilerplate where the assay design is adjudicable. Restored relevant verbatim biochemical and localization evidence; did not restore label-only CHAIN features, unrelated introductory statements, or quotations that no longer support the revised claim. Existing source annotation fields were preserved.