Sources: UniProt P35579, cached GOA-cited publications (many abstract-only), PMID:37987147 (amoeboid nucleokinesis).
Falcon deep research was not available when the review was finalized.
Core biology
- Heavy chain of non-muscle myosin IIA; actin-activated Mg-ATPase that translocates actin
PMID:12237319.
- Forms bipolar filaments after RLC phosphorylation PMID:24072716.
- Contractile ring / cleavage furrow PMID:11029059.
- Only class II myosin in T cells; uropod and motility PMID:15064761.
Nucleokinesis (module context)
- MYH9-GFP accumulates behind the nucleus during amoeboid (dendritic cell) nucleokinesis; myosin II forces drive it
PMID:37987147.
- The neuronal nucleokinesis papers used by the module are either paralog-agnostic (blebbistatin) or MYH10-specific
(PMID:19607793 studies Myosin IIB in cerebellar granule neurons). No MYH9-specific neuronal evidence found in cache,
so no NEW nuclear migration annotation was added.
Citation problem
- PMID:2732579 (cited by GOA for RAB3A binding, lysosome localization, regulated exocytosis, regulation of plasma
membrane repair) is a 1989 Japanese case report on hypoparathyroidism. Same WITH/FROM partner (RAB3A) and content as
PMID:27325790 (Rab3a/Slp4-a/NMHC-IIA), whose id it truncates. Recorded as WRONG_IDENTIFIER with replacement.
Decisions summary
- Protein binding: S100A4/S100 papers -> MODIFY to GO:0044548 S100 protein binding; RAB3A -> GO:0031267 small GTPase binding;
ITGB3 -> integrin binding; the rest REMOVE (uninformative).
- ACE shedding (membrane protein ectodomain proteolysis) -> MODIFY to negative regulation (GO:0051045).
- Exosome/RNA-binding/COP9/cadherin HDA rows -> MARK_AS_OVER_ANNOTATED.
- Nucleus (PMID:14508515) -> UNDECIDED (abstract-only, cannot see nuclear data).