Gene Ontology annotation through association of InterPro records with GO terms

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:21261884

Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440.

PP_0421 is trpD encoding anthranilate phosphoribosyltransferase; a transposon insertion at codon 184 of trpD causes tryptophan auxotrophy, and trpD lies in a trpGDC operon. The trpD mutant is rescued by L-tryptophan and indole.

PMID:20158506

Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome-wide mutant library.

A trpD mutant is auxotrophic on M9 minimal medium with growth restored by L-tryptophan, confirming trpD is required for endogenous tryptophan biosynthesis.