Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Towards a proteome-scale map of the human protein-protein interaction network.
Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex.
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The PML-body localization reported by this paper is for SAP25, not LRCH4.
"A fraction of SAP25 is located in promyelocytic leukemia protein (PML) nuclear bodies, and PML induces a striking nuclear accumulation of SAP25."
An empirical framework for binary interactome mapping.
Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.
A proteome-scale map of the human interactome network.
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.
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The study systematically characterized RhoGEF/RhoGAP interaction networks and subcellular localization as spatial organizers of Rho signaling.
"Through a family-wide characterization of substrate specificities, interactomes and localization, we reveal at the systems level how RhoGEFs and RhoGAPs contextualize and spatiotemporally control Rho signalling."
A reference map of the human binary protein interactome.
Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes.
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The chromosome 7q22 sequencing study identified the LRCH4 locus as a novel gene with weak sequence similarity to a neuronal leucine-rich-repeat protein.
"Two genes showed weak similarity to an insulin-like receptor and a neuronal protein with a leucine-rich amino-terminal domain."
Leucine-rich repeats and calponin homology containing 4 (Lrch4) regulates the innate immune response.
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GFP-tagged murine Lrch4 expressed in engineered HEK293 cells showed substantial plasma-membrane overlap, especially at low-to-medium expression.
"Microscopy of GFP-Lrch4 in HEK293 cells reveals staining that includes a cytoplasmic-type pattern, but formal analysis indicates a high degree of overlap with the plasma membrane stain CellMask TM (Life Technologies) ( 22 ), in particular in cells with low-medium forced expression, consistent with substantial localization to the plasma membrane ( Fig. 1 E )."
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Mouse Lrch4 depletion attenuated cytokine induction by LPS and several other TLR ligands and reduced the innate response in vivo.
"Silencing Lrch4 attenuates cytokine induction by LPS and multiple other TLR ligands and dampens the in vivo innate immune response."
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Mouse Lrch4 promotes delivery of LPS into lipid-raft membrane microdomains.
"Lrch4 promotes proper docking of LPS in lipid raft membrane microdomains."
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Mouse Lrch4 depletion reduced cell-surface gangliosides and expression/surface display of the raft-resident LPS co-receptor CD14.
"We provide evidence that this is through regulation of lipid rafts as Lrch4 silencing reduces cell surface gangliosides, a metric of raft abundance, as well as expression and surface display of CD14, a raft-resident LPS co-receptor."
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Depletion of endogenous human LRCH4 attenuated TLR2- and TLR4-dependent IL-8 induction in engineered HEK293 cells without reducing the response to TNF-alpha.
"As shown in Fig. 2 G , Lrch4 siRNA attenuated IL-8 induction by both ligands in HEK293 cells, providing further support that the shRNA results in macrophages are unlikely to reflect off-target effects."
Impact of Escherichia coli K12 and O18:K1 on human platelets: Differential effects on platelet activation, RNAs and proteins.
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Human platelet LRCH4 RNA decreased after exposure to E. coli K12, with the change detected independently by two RNA-sequencing pipelines.
"For the RNAs of HMBS (logFC = +5.73), ATP2C1 (logFC = -3.13) and LRCH4 (logFC = -4.07) changes were detectable by thromboSeq and Tuxedo pipelines."
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LRCH4 RNA and protein were detected in human platelets, while E. coli exposure did not visibly change LRCH4 protein abundance or molecular weight.
"By Western blot analysis we could demonstrate the presence of HMBS, ATP2C1 and LRCH4 proteins in platelets before and after E. coli K12 exposure"
Knockdown of LRCH4 Remodels Tumor Microenvironment Through Inhibiting YAP and TGF-β/Smad Signaling Pathway in Colorectal Cancer.
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LRCH4 knockdown inhibited proliferation, migration and invasion of human HT29 colorectal-cancer cells.
"Here, we reported that the knockdown of LRCH4 inhibited the proliferation, migration and invasion in HT29 cells."
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LRCH4 knockdown reduced YAP activity, reversed EMT-associated features and inhibited TGF-beta/Smad signaling in HT29 cells.
"The activity of Yes-Associated Protein (YAP), a transcription factor in the Hppo-YAP signaling pathway, was significantly inhibited by LRCH4-siRNA. LRCH4 knockdown also reversed the EMT and regulated the expression of extracellular matrix (ECM) protein, Fibronectin and Collagen IV in HT29 cells. In addition, the TGF-β/Smad signaling pathway, as the downstream pathway of Yap, was also inhibited by LRCH4 knockdown."