SHY1 has two IBA rows from GO_REF:0000033, both traced by GOA to
PANTHER:PTN000603741 in the SURF1/Shy1 family. I fetched the current
PTHR23427 metadata, entries table, and PAINT node export; the cached PAINT
slice still places both GO:0033617 mitochondrial respiratory chain complex IV
assembly and GO:0005739 mitochondrion on PTN000603741, and
S. cerevisiae Shy1 is in PTHR23427:SF2 SURFEIT LOCUS PROTEIN 1.
The complex IV assembly IBA is a sound core transfer. S. cerevisiae Shy1 has
direct experimental support as a complex IV assembly factor that interacts with
Mss51, Cox14, Coa1, and complex IV subcomplexes [PMID:17882259; PMID:17882260],
and the current review already captures this as SHY1's central process. The
mitochondrion IBA is also sound, with direct inner-mitochondrial-membrane
evidence from the original yeast characterization PMID:9162072. The 2024
S. pombe Shy1/SURF1 homolog study provides newer ortholog corroboration for
mitochondrial localization and complex IV assembly, but it also reports that
S. pombe shy1 deletion does not critically disrupt respiratory-chain assembly
PMID:39289458. I
therefore treated it as corroborating ortholog context rather than direct
S. cerevisiae evidence.
I reclassified both GO:0005515 protein binding IPI rows from
MARK_AS_OVER_ANNOTATED to REMOVE under the current generic-protein-binding
policy. Both current IntAct rows are Shy1-Coa1 edges; that contact is real, but
the informative curation is Shy1's role in complex IV assembly, not a standalone
protein-binding MF. I changed the obsolete GO:0051082 unfolded protein binding
IMP row to MODIFY with a GO:0140777 protein-containing complex stabilizing
activity replacement; PMID:11389896 supports a Shy1-containing assembly complex
and a complex IV assembly/stability role, not general binding to unfolded
substrates.
I searched 2023-2026 SHY1/Shy1/SURF1 yeast literature. I did not find a newer
direct S. cerevisiae SHY1 paper. The relevant recent paper is Luo et al. 2024 on
the S. pombe SURF1 homolog Shy1 PMID:39289458, which is useful for IBA
ortholog context because PomBase:SPBC1215.01 appears in the GOA
WITH/FROM field for the mitochondrial IBA.
Refreshing GOA added updated exact-source IBA rows for GO:0005739
mitochondrion and GO:0033617 mitochondrial respiratory chain complex IV
assembly, both still traced to the same SURF1-family PAINT node,
PANTHER:PTN000603741. The 2026-10-01 PAINT export adds the mouse MGI:MGI:98443
seed to both rows while retaining direct S. cerevisiae SHY1 support and the
same inherited mitochondrial-localization and complex-IV-assembly calls, so I
accepted the refreshed rows and marked the previous no-MGI/no-qualifier
duplicates as retired provenance.
The current GOA export has also withdrawn the GO:0051082 unfolded protein
binding IMP row from PMID:11389896. I left that older MODIFY review in place
with retired: true because the literature still supports a Shy1 role in
stabilizing complex IV assembly intermediates rather than a broad
unfolded-protein-binding activity.