Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Dual-substrate specificity short chain retinol dehydrogenases from the vertebrate retina.
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RDH12 is one of four novel SDR retinol dehydrogenases (RDH11-14) with dual-substrate specificity, metabolizing all-trans- and cis-retinols with C15 pro-R specificity; source of the IDA molecular-function annotations.
"four retinol dehydrogenases (RDH11-14) that display dual-substrate specificity, uniquely metabolizing all-trans- and cis-retinols"
Biochemical properties of purified human retinol dehydrogenase 12 (RDH12): catalytic efficiency toward retinoids and C9 aldehydes and effects of cellular retinol-binding protein type I (CRBPI) and cellular retinaldehyde-binding protein (CRALBP) on the oxidation and reduction of retinoids.
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Purified human RDH12 strongly prefers NADP(H) over NAD(H) (~2000-fold lower Km), is most efficient for all-trans-retinal, and in most tissues primarily reduces all-trans-retinaldehyde; localizes to the ER membrane.
"RDH12 exhibits approximately 2000-fold lower K(m) values for NADP(+) and NADPH than for NAD(+) and NADH"
Retinol dehydrogenase 12 detoxifies 4-hydroxynonenal in photoreceptor cells.
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In mouse retina RDH12 reduces the lipid-peroxidation aldehyde 4-HNE to a nontoxic alcohol in photoreceptor inner segments, protecting against light-induced apoptosis; its visual-cycle contribution is indirect.
"in mouse retina RDH12 reduces 4-HNE to a nontoxic alcohol, protecting cellular macromolecules against oxidative modification and protecting photoreceptors from light-induced apoptosis"
Disease-associated variants of microsomal retinol dehydrogenase 12 (RDH12) are degraded at mutant-specific rates.
A proteome-scale map of the human interactome network.
Widespread macromolecular interaction perturbations in human genetic disorders.
A reference map of the human binary protein interactome.
The canonical retinoid cycle in rods (twilight vision)
RDH12 reduces atRAL to atROL
Defective RDH12 does not reduce atRAL to atROL