Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid.
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Zebrafish pol beta was purified and its DNA polymerase kinetics measured.
"We investigated the kinetic constants Km and kcat, and the catalytic efficiency kcat/Km for Danio pol β in the presence of either Mg2+ or Mn2+."
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Catalytic efficiency is 8.6-fold higher with Mn2+ than with Mg2+.
"Substituting Mn2+ for Mg2+ resulted in 8.6-fold higher catalytic efficiency (kcat/Km)."
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The zebrafish-specific P63D substitution lies in the 8-kDa domain hairpin implicated in DNA binding.
"This substitution lies in a hairpin sequence within an 8-kDa domain, likely to be important in DNA binding."