Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Cloning, expression in Escherichia coli, and characterization of Arabidopsis thaliana UMP/CMP kinase
Evolution of the chalcone-isomerase fold from fatty-acid binding to stereospecific catalysis
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CHI superfamily includes non-catalytic fatty acid-binding proteins (FAPs) that localize to plastids
"These three CHI-fold proteins localize to plastids, the site of de novo fatty-acid biosynthesis in plant cells. Furthermore, their expression profiles correlate with those of core fatty-acid biosynthetic enzymes"
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FAPs are non-enzymatic ancestors of catalytically active chalcone isomerases
"the FAP discovery defines the adaptive evolution of a stereospecific and catalytically 'perfected' enzyme from a non-enzymatic ancestor over a defined period of plant evolution."
UMP kinase activity is involved in proper chloroplast development in rice
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Rice YL2 is a thylakoid membrane-localized UMP kinase essential for chloroplast development
"YL2 encodes a thylakoid membrane-localized protein with significant sequence similarity to UMP kinase proteins in prokaryotes and eukaryotes. Prokaryotic UMP kinase activity was subsequently confirmed, with YL2 deficiency causing a significant reduction in chlorophyll accumulation and photochemical efficiency."
PUMPKIN, the Sole Plastid UMP Kinase, Associates with Group II Introns and Alters Their Metabolism
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PUMPKIN is the sole plastid UMP kinase in Arabidopsis
"the sole plastid UMP kinase (PUMPKIN) in Arabidopsis (Arabidopsis thaliana) associates specifically with the introns of the plastid transcripts trnG-UCC, trnV-UAC, petB, petD, and ndhA in vivo"
Chromosome-scale assembly and analysis of biomass crop Miscanthus lutarioriparius genome
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M. lutarioriparius genome has 68,328 predicted gene models, unusually high even for an allotetraploid
"A total of 68,328 gene models were predicted, with an average CDS length of 1215 bp"