Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UniProt entry Q17778 (NUC1_CAEEL)
An endonuclease from Caenorhabditis elegans: partial purification and characterization.
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The major C. elegans DNase is an EDTA-insensitive endonuclease that prefers dsDNA, and nuc-1 mutations reduce it to below 1% of wild type.
"Three independent mutations in the nuc-1 gene were shown to reduce nuclease activity to less than 1% of that seen in wild-type organisms."
NUC-1, a caenorhabditis elegans DNase II homolog, functions in an intermediate step of DNA degradation during apoptosis.
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nuc-1 encodes a DNase II homolog that converts TUNEL-reactive apoptotic DNA to a TUNEL-unreactive state, and completion of degradation needs engulfment.
"Mutations in nuc-1 allowed the generation of TUNEL-reactive DNA but blocked the conversion of TUNEL-reactive DNA to a subsequent TUNEL-unreactive state."
The C. elegans apoptotic nuclease NUC-1 is related in sequence and activity to mammalian DNase II.
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C07B5.5 encodes NUC-1. Mutant extracts lose DNase II-like endonuclease activity, and the transgene complements.
"wild-type animals contained a potent endonuclease activity with a cleavage preference similar to DNase II, while nuc-1 mutant worms demonstrated a marked reduction in this nuclease activity"
The roles and acting mechanism of Caenorhabditis elegans DNase II genes in apoptotic dna degradation and development.
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NUC-1 is the major DNase II for apoptotic DNA degradation and the only one for bacterial DNA in the intestine. It is secreted and acts across its expression border.
"Removal or disruption of NUC-1 secretion signal eliminates NUC-1's ability to mediate DNA degradation across its expression border."
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The authors propose NUC-1 can act in apoptotic cells independently of engulfment. This is disputed by Wu et al. 2000 and by a 2026 preprint.
"blocking cell corpse engulfment does not affect apoptotic DNA degradation mediated by nuc-1, suggesting that NUC-1 acts in apoptotic cells rather than in phagocytes to resolve 3' OH DNA breaks"
Sequential action of Caenorhabditis elegans Rab GTPases regulates phagolysosome formation during apoptotic cell degradation.
LAAT-1 is the lysosomal lysine/arginine transporter that maintains amino acid homeostasis.
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NUC-1::mCHERRY labels lysosomes and phagolysosomes in C. elegans.
"Using NUC-1::mCHERRY, which labels lysosomes (8, 9)"
Autonomous and non-autonomous roles of DNase II during cell death in C. elegans embryos.
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ToLFP detects DNase II cleavage products. NUC-1 is the major embryonic DNase II and acts mainly non-autonomously and in a secretion-dependent manner.
"anteriorly expressed NUC-1 digests apoptotic DNA in posterior blastomeres in a non-autonomous and secretion-dependent manner"
Loss of DNase II function in the gonad is associated with a higher expression of antimicrobial genes in Caenorhabditis elegans.
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NUC-1 degrades apoptotic germ cell DNA. Its loss raises antimicrobial gene expression, probably through ERK activation by undigested DNA.
"nuc-1 and crn-7 play a role in degrading apoptotic DNA in distinct sites of the gonad"
Nematodes Degrade Extracellular Antibiotic Resistance Genes by Secreting DNase II Encoded by the nuc-1 Gene.
pH-gated activation of nematodes-secreted NUC-1 accelerates extracellular antibiotic resistance gene degradation in aquatic environments.
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Secreted NUC-1 activity is gated by pH, with maximal activity at pH 6.
"environmental pH specifically modulates NUC-1 activity rather than expression"
WormBase gene description for nuc-1 (worm)