Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Gene Ontology annotation through association of InterPro records with GO terms
Loss of αBa-crystallin, but not αA-crystallin, increases age-related cataract in the zebrafish lens.
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cryaba null fish develop more lens opacity than cryaa nulls by 24 months.
"loss of the αBa-crystallin gene cryaba led to an increase in lens opacity compared to cryaa null fish at 24 months of age"
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cryaba is the leading alpha-crystallin protecting against age-related cataract.
"lens-specific zebrafish αBa-crystallin plays the leading role in preventing age-related cataract"
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The protective role of cryaba grows in importance during lens aging.
"the protective role for cryaba becomes more important during lens aging"
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No compensatory upregulation of the other crystallins occurs in the mutants.
"None of the three α-crystallin mutants showed a compensatory increase in the expression of the remaining two crystallins"
Zebrafish alpha-crystallins: protein structure and chaperone-like activity compared to their mammalian orthologs.
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Chaperone-like activity was assayed as prevention of chemically induced aggregation between 25 and 40 degrees C.
"The chaperone-like activities of zebrafish and human alpha-crystallins were compared by assaying their ability to prevent the chemically induced aggregation of several target proteins at temperatures between 25 degrees C and 40 degrees C"
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Zebrafish alphaB-crystallin is a much weaker chaperone than zebrafish alphaA-crystallin.
"The chaperone-like activities of the two zebrafish alpha-crystallins were highly divergent, with alphaA-crystallin showing much greater activity than alphaB-crystallin"
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Reduced chaperone function and lack of extralenticular expression indicate a different physiological role from the mammalian ortholog.
"The reduced chaperone-like function of zebrafish alphaB-crystallin and its lack of extralenticular expression indicates that it plays a different physiological role from its mammalian ortholog"
Gene duplication and separation of functions in alphaB-crystallin from zebrafish (Danio rerio).
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Zebrafish alphaB1-crystallin is not constitutively expressed in nervous or muscular tissue and has reduced chaperone-like activity.
"not constitutively expressed in nervous or muscular tissue and has reduced chaperone-like activity"
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After duplication alphaB2 kept the widespread protective role while alphaB1 took a restricted lens role.
"while zebrafish alphaB1 adopted a more restricted, nonchaperone role in the lens"
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Zebrafish is the first species known to express two alphaB-crystallins.
"Zebrafish is the first species known to express two different alphaB-crystallins"
In vivo characterization of human myofibrillar myopathy genes in zebrafish.
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Human myofibrillar myopathy genes were knocked down in zebrafish and muscle and cardiac phenotypes scored.
"we here specifically inactivated known human MFM disease genes and evaluated the resulting muscular and cardiac phenotypes functionally and structurally"
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Knockdown compromised skeletal muscle function through myofibrillar degeneration and caused heart failure.
"targeted ablation of MFM genes in zebrafish led to compromised skeletal muscle function mostly due to myofibrillar degeneration as well as severe heart failure"
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Phenotypes differed between the genes tested.
"MFM gene-deficient zebrafish showed pronounced gene-specific phenotypic and structural differences"
Lengsin expression and function during zebrafish lens formation.
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Lengsin expression follows the temporal expression of alphaA-, alphaB1- and betaB1-crystallin proteins in the lens.
"Lengsin expression in zebrafish follows the temporal expression of the alphaA- alphaB1- and betaB1-crystallin proteins in the lens"
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In the adult lens the protein studied is restricted to cortical fibres and co-localizes with actin at lateral cell faces.
"In the adult lens, Lengsin is restricted to a narrow band of cortical fibers and co-localizes with actin at the lateral faces of these interdigitating cells"
UniProtKB entry Q9PUR2 for Danio rerio cryaba
Falcon (Edison) deep research report: zebrafish cryaba (Q9PUR2) functional annotation of alphaBa-crystallin
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Lens proteomics detected an alphaBa-crystallin peptide in wild-type adult lenses that was absent in cryaba null lenses, confirming the gene product is a lens protein in vivo.
"adult lens proteomics (parallel reaction monitoring) detected an **αBa‑crystallin peptide** in wild type lenses that was **absent in cryaba−/−** lenses"