Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Functional Annotation of a Presumed Nitronate Monoxygenase Reveals a New Class of NADH:Quinone Reductases.
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Purified PA1024 has no nitronate monooxygenase activity. It has NADH:quinone reductase activity and a small NADH oxidase activity.
"Purified PA1024 did not exhibit nitronate monooxygenase activity; however, it displayed NADH:quinone reductase and a small NADH:oxidase activity."
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The enzyme prefers NADH to NADPH and reduces many quinones by a Ping Pong Bi Bi mechanism.
"PA1024 could reduce a broad spectrum of quinone substrates via a Ping Pong Bi Bi steady-state kinetic mechanism, generating the corresponding hydroquinones."
Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base.
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Crystal structures of PA1024 bound to FMN, alone and with 2-nitropropane. The paper's assumption of nitronate monooxygenase activity was later refuted (PMID:27502282).
"a binary complex with FMN and a ternary complex with both FMN and 2-nitropropane"
UniProt entry Q9I4V0 (NQRED_PSEAE)