Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
A global control region defines a chromosomal regulatory landscape containing the HoxD cluster.
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Lnp shares limb and CNS expression with neighboring Hoxd genes and Evx2, but the study does not test a causal limb function.
"During limb development, coordinated expression of several Hoxd genes is required in presumptive digits. We searched for the underlying control sequences upstream from the cluster and found Lunapark (Lnp), a gene which shares limb and CNS expression specificities with both Hoxd genes and Evx2, another gene located nearby."
ER network formation requires a balance of the dynamin-like GTPase Sey1p and the Lunapark family member Lnp1p.
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Human Lnp expressed in mammalian cells localizes to ER three-way junctions; most mechanism in the paper is yeast evidence.
"In mammalian cells, Lnp1 also localized to the three way junctions of the ER (Fig. 2e), suggesting that its function is conserved from yeast to higher cells."
Protein N-myristoylation plays a critical role in the endoplasmic reticulum morphological change induced by overexpression of protein Lunapark, an integral membrane protein of the endoplasmic reticulum.
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Human LNPK is a double-spanning integral ER membrane protein with cytosolic termini.
"Analysis of tumor necrosis factor-fusion proteins with each of the two putative transmembrane domains and their flanking regions of protein Lunapark revealed that transmembrane domain 1 and 2 functioned as type II signal anchor sequence and stop transfer sequence, respectively, and together generated a double-spanning integral membrane protein with an N-/C-terminal cytoplasmic orientation."
A model for the generation and interconversion of ER morphologies.
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A curvature model and mammalian experiments support LNPK stabilization of ER three-way junctions.
"Lunapark is an example of S-type proteins that promote junctions between tubules and sheets. In a tubular ER network, lunapark stabilizes three-way junctions, i.e., small triangular sheets with concave edges."
Lunapark stabilizes nascent three-way junctions in the endoplasmic reticulum.
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Mammalian live imaging shows that LNPK-positive nascent ER junctions remain stable while LNPK-negative junctions close.
"Newly formed junctions that acquire mLnp1 remain stable within the ER network, whereas nascent junctions that fail to acquire mLnp1 undergo rapid ring closure."
Cooperation of the ER-shaping proteins atlastin, lunapark, and reticulons to generate a tubular membrane network.
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Human U2OS LNPK knockout shifts peripheral ER from tubules and junctions toward sheets, and low-level rescue restores the reticular network.
"We generated a CRISPR knock-out of lunapark in U2OS cells that express GFP-calreticulin (Figure 8—figure supplement 1A). Cells lacking Lnp exhibited a proliferation of peripheral sheets and a reduction of tubules and junctions (Figure 8A; quantification in Figure 8B)."
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The zinc-finger-containing region is required for LNPK self-interaction and junction targeting.
"Taken together, these results show that localization to three-way junctions requires several Lnp features, including the Zn2+-finger implicated in dimerization."
Mutations in LNPK, Encoding the Endoplasmic Reticulum Junction Stabilizer Lunapark, Cause a Recessive Neurodevelopmental Syndrome.
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Human biallelic LNPK loss-of-function causes a neurodevelopmental syndrome with corpus-callosum hypoplasia and abnormal ER structure.
"Affected cells showed the absence of full-length lunapark, aberrant ER structures, and increased luminal mass density. Together, our results implicate the ER junction stabilizer lunapark in establishing the corpus callosum."
A reference map of the human binary protein interactome.
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HuRI supplies binary interaction candidates, but their cellular functions generally remain unresolved.
"While HuRI displays highly significant overlap with known functional relationships, the cellular function of most individual PPIs remains to be elucidated."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex generated cell-line-specific human AP-MS interaction networks.
"Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks."
Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions.
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Mammalian-cell purification reports associated and intrinsic in-vitro ubiquitin-ligase activity for LNPK.
"Accordingly, Lnp purified from mammalian cells has a ubiquitin ligase activity in vitro Intriguingly, biochemical analyses show that this activity can be attributed not only to associated ubiquitin ligase, but also to an intrinsic ubiquitin ligase activity borne by Lnp itself."
Reciprocal regulation between lunapark and atlastin facilitates ER three-way junction formation.
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LNPK and atlastin reciprocally regulate ER-junction formation, with LNPK stabilizing rather than catalyzing membrane fusion.
"Three-way junctions are characteristic structures of the tubular endoplasmic reticulum (ER) network. Junctions are formed through atlastin (ATL)-mediated membrane fusion and stabilized by lunapark (Lnp)."
Secretome translation shaped by lysosomes and lunapark-marked ER junctions.
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Human-cell LNPK loss impairs eIF2-dependent secretome-mRNA initiation at lysosome-proximal ER junctions.
"Lunapark depletion reduced ribosome density and translation efficiency of secretome mRNAs near lysosomes, an effect that was dependent on eIF2-mediated initiation and was reversed by the integrated stress response inhibitor ISRIB."
UniProtKB Q9C0E8 (LNPK_HUMAN) reviewed entry