title: DSPP source and assay guide
gene: DSPP
annotation_scope:
imported_annotations: 15
new_annotations: 0
This guide identifies the experimental source behind each biological context. Repository cache flags describe stored content; separately accessible original sections and their limits are recorded below. No figure-pixel inspection is claimed for these sources.
| Source | Actual original access and experimental boundary |
|---|---|
| 8995371 — author original | Original Methods, Results, Discussion and captions read. Mouse full cDNA, rat sequence correction and partial human chromosome-mapping probe have different roles. Normal cache: abstract only. |
| 9879917 — publisher | Authentic abstract and indexed original pages 1043/1044/1046, including human molar cloning context. Complete original unavailable; no new direct human binding experiment claimed. Normal cache: abstract only. |
| 16046405 — author original | Operative Methods/Results/Discussion and captions read. Rat native phosphophoryn versus a shorter rat recombinant C-terminal fragment, with calcium, collagen and mineral assays. Normal cache: abstract only. |
| 40640300 | Full normal XML; mouse genotype comparisons, histology, matrix/mineral Results and Methods read. The DPP truncation and null resolve later-stage contributions; no supplement or isolated human-product assay claimed. |
| 22615197 | Full normal XML; native rat incisor DPP isolation, radiolabeled substrate adsorption and adhesion controls read. Collagen association is directly measured. |
| 11856645 | Complete authentic human-tooth abstract. Full intracellular localization panel and detailed antibody Methods unavailable. Other proteins in the same abstract are not treated as DSPP evidence. |
| 19001636 | Indexed original mouse Methods, antisera/controls, Figure 8/9 captions and Discussion read. Normal cache: abstract only. |
| 23525114 | Indexed original rat RUB1 localization Results and Figure 5/6 captions/controls read. Normal cache: abstract only. |
| 15763925 | Full normal XML; rat M2H4 expression/mineralization and TGFbeta/BMP4 treatment Methods and Results read. This is expression-marker evidence, not a DSPP perturbation. |
| 24502800 | Complete authentic abstract for the rat IEP donor context; no full original or unseen direct perturbation claimed. |
| 25027178 | Selected indexed original mouse WT/null Methods, operative lineage-marker Results and Discussion read. Normal cache: abstract only. |
| 22134916 — author original | Selected original Methods/Results and captions: mouse C3H10T1/2 and primary mouse pulp cells, recombinant DPP substrate, integrin/FAK/ERK controls and differentiation endpoints. Exact reagent sequence/species unresolved. Normal cache: abstract only. |
| 15308669 — publisher | Authentic abstract and selected original promoter-study Methods. Complete paper unavailable; exact attribution of the donor protein-to-process annotation remains unresolved. |
| 36399478 | Existing full XML; resource Methods, matrix classes and aggregate coverage Results. No DSPP-specific mass-spectrometry row independently verified. |
The official human sequence and features distinguish the 1301-residue primary sequence from the signal peptide and processed chains. The PTHR47819 topology, family alignment and PAINT IBD file provide the literal lineage evidence. Select human leaf PTN000789080 and its ancestor PTN002936180, then the five IBD records for GO:0005518, GO:0005634, GO:0005737, GO:0071895 and GO:0097187. The human path has eight nodes including the root and leaf; it is not inferred from donor similarity alone.
Current donor annotations are queryable for rat Q62598 and mouse P97399. The captured rat record has 49 rows and mouse 39. The annotation references differ by term; the rat marker and regulatory-source limitations are stated in the review. Live services may evolve after this review.
The target-resolved pathway chain is reaction R-HSA-4086132 → extracellular DPP R-HSA-4086140, with integrin alphaVbeta1 R-HSA-216015 and product complex R-HSA-4086137. Nonhuman inferred entity coordinates are not substituted for experimentally authenticated rodent sequences. No target activity was present in the inspected GO-CAM index; that absence alone is not an annotation gap.
The more specific core location GO:0140149 is a cellular-component class. It is distinct from the molecular function GO:0005201. Calcium/collagen interactions are integrated in that matrix mechanism; contextual intracellular or developmental assertions do not each require a separate core.
| Source | Operative evidence and public locator | Boundary |
|---|---|---|
| PMID:18521831(https://pmc.ncbi.nlm.nih.gov/articles/PMC5534847/) | Methods: Kindreds and Genomic DNA, DSPP Mutation Analysis in Early Exons, Cloning and Sequencing of DSPP Repeat Domain; Results: Patient Description and Mutational Analysis; Discussion. Human family sequencing, segregation and control chromosomes. | Human disease genetics supports dentin relevance; it does not measure calcium/collagen chemistry. Frameshift position versus phenotype is a reported trend, not an invariant rule. |
| PMID:22392858(https://pmc.ncbi.nlm.nih.gov/articles/PMC3390460/) | Methods: Expression Constructs, Cell culture and transfection, Quantitative ELISA and Immunocytochemistry; Results: Secretion of WT DSPP is reduced when co-expressed with mutant DSPP proteins, Figure 6. Both original full text and an unmodified publication cache were acquired. | The first 31 human residues were fused to mouse DSPP or GFP, with additional engineered frameshift models in HEK293A. Reduced normal-protein secretion supports dominant-negative trafficking in this system. Neither complete natural human chemistry nor a normal nuclear role is demonstrated. The calcium-aggregation mechanism was not directly reconstituted. |
The original source identifiers for all annotations remain intact. The four withdrawn functional-support links are historical precursor/sequence/promoter sources; their removal from particular support lists does not allege misidentification or prove the complete papers lack relevant experiments. In particular, PMID:15308669 remains a verified bibliographic/promoter-context reference with unresolved source-specific regulatory attribution. PMID:36399478 remains a verified resource reference without a verified DSPP measurement. The existing nuclear and cytoplasmic PAINT/source paths above remain the basis of the positive non-core decisions, independently of disease-mutant retention.