Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP.
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DdPDE1 (PdsA) is a class II dual-specificity phosphodiesterase that degrades extracellular cAMP and cGMP and can be secreted or displayed on the cell surface.
"DdPDE1 (PdsA) is a class II dual-specificity PDE that degrades extracellular cAMP and cGMP. The enzyme can be secreted in the medium, or exposed on the cell surface."
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PdsA is the main enzyme degrading extracellular cAMP during aggregation and is essential for shaping cAMP waves; pdsA-null (UK7) cells fail to aggregate.
"DdPDE1 is the main PDE that degrades extracellular cAMP during cell aggregation and is thereby essential for shaping cAMP waves"
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PdsA and its homologue DdPDE7 have dual cAMP/cGMP specificity and are strongly inhibited by DTT and insensitive to IBMX.
"Both DdPDE1 and DdPDE7 are PDEs with dual cAMP/cGMP specificity, and are strongly inhibited by DTT and insensitive to IBMX."
The group migration of Dictyostelium cells is regulated by extracellular chemoattractant degradation.
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The intracellular pool of PdsA is localized to the endoplasmic reticulum, proposed as a storage/secretion compartment.
"the intracellular pool of PdsA is localized to the endoplasmic reticulum, which may provide a compartment for storage and secretion of PdsA"
Cyclic nucleotide phosphodiesterase of Dictyostelium discoideum and its glycoprotein inhibitor: structure and expression of their genes.
Conditions that alter intracellular cAMP levels affect expression of the cAMP phosphodiesterase gene in Dictyostelium.
Binding of inhibitor alters kinetic and physical properties of extracellular cyclic AMP phosphodiesterase from Dictyostelium discoideum.
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PdsA forms a complex with a heat-stable glycoprotein inhibitor that raises its Km; dithiothreitol relieves inhibition by inactivating the inhibitor.
"Treating the enzyme-inhibitor complex with dithiothreitol stimulated enzyme activity 20- to 100-fold"
The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.
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EGCG delays aggregation and stalls development at the loose aggregate stage.
"In the presence of EGCG aggregation is delayed, cells do not stream and development is typically stalled at the loose aggregate stage."
Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.
The cyclic nucleotide specificity of eight cAMP-binding proteins in Dictyostelium discoideum is correlated into three groups.
The extracellular cyclic nucleotide phosphodiesterase of Dictyostelium discoideum. Purification and characterization.
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Two forms of the secreted extracellular cyclic nucleotide phosphodiesterase were purified from Dictyostelium.
"Two forms of the extracellular cyclic nucleotide phosphodiesterase (EC 3.1.4.17) of Dictyostelium discoideum have been purified."
Purification and characterization of the extracellular cyclic AMP phosphodiesterase of Dictyostelium discoideum.
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The extracellular cAMP phosphodiesterase was purified from aggregation-phase culture supernatant.
"Extracellular phosphodiesterase for adenosine 3':5'-monophosphate [EC 3.1.4.17] was purified from the supernatant of aggregation phase culture of Dictyostelium discoideum"
The phosphodiesterase secreted by prestalk cells is necessary for Dictyostelium morphogenesis.
Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.
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The cell-surface phosphodiesterase localizes to plasma membrane microdomains together with cAR1 and adenylate cyclase.
"The cell surface phosphodiesterase (PDE) and a downstream effector of cAR1, adenylate cyclase (ACA), were specifically localized in these structures"
Robustness of self-organizing chemoattractant field arising from precise pulse induction of its breakdown enzyme: a single-cell level analysis of PDE expression in Dictyostelium.
Null mutations of the Dictyostelium cyclic nucleotide phosphodiesterase gene block chemotactic cell movement in developing aggregates.
Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP.
Multiple phosphorylation sites on the RegA phosphodiesterase regulate Dictyostelium development.
Regulated protein degradation controls PKA function and cell-type differentiation in Dictyostelium.
PdsA/DdPDE1 (DICDI, UniProt P12019) — Adjudication of two negative-regulation GO claims