Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli.
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Genetic analysis established PurN and PurT as alternative enzymes at the third de novo purine-biosynthesis step.
"We demonstrate here that Escherichia coli synthesizes two different glycinamide ribonucleotide (GAR) transformylases, both catalyzing the third step in the purine biosynthetic pathway."
UniProtKB entry Q88MB0 for Pseudomonas putida KT2440 purN
OpenScientist gene research for PSEPK purN
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The report corroborates the PurN reaction and the parallel PurT route while explicitly distinguishing orthology-based inference from direct KT2440 characterization.
"No study to date has biochemically or genetically characterized the *P. putida* KT2440 purN gene product specifically."