Exact emitted record, retrieved 2026-09-10T14:34:29.656765+00:00.
Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins
NPI (CS 0; DOMAIN_ARCHITECTURE_MISMATCH) for “binds GTP” in the selected G3V4H1 product; UNC (CS 1) for “lacks intrinsic GTPase activity and is resistant to Rho-specific GAPs”. GTP binding is true of the RHOJ gene, but guanine-nucleotide binding needs the G4 (TQID) and G5 (CSAL) guanine-base motifs, and G3V4H1 keeps only the P-loop, switch I and switch II before diverging after residue 103 into a tail read from the canonical 3′ UTR. The “lacks hydrolysis, resists GAPs” description is contradicted for full-length RHOJ by direct hydrolysis measurements; for this incomplete G-domain product no binding, hydrolysis or GAP assay exists, so that part can be neither confirmed nor refuted. PMID:10967094(https://pubmed.ncbi.nlm.nih.gov/10967094/); sequence mapping; G-domain motif comparison.
The assessment concerns the selected accession G3V4H1. Other emitted outputs and evidence context.