Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
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SwissProt keyword-derived (SPKW) annotations present in the Sept 2025 goa_uniprot_gcrp snapshot but removed from the current GOA release after GOA retired the keyword2GO pipeline for cellular organisms.
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For OsEIL2, the keyword "Ethylene signaling pathway" mapped to a genuinely correct, core process term (GO:0009873 ethylene-activated signaling pathway); its removal is collateral damage, not a correction, because EIN3/EIL transcription factors are defining components of the ethylene-activated signaling pathway.
Electronic Gene Ontology annotations created by ARBA machine learning models
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ARBA models assign sequence-specific DNA binding, positive regulation of DNA-templated transcription and regulation of the ethylene-activated signaling pathway to OsEIL2, all consistent with experimental data for this EIN3/EIL transcription factor.
Combined Automated Annotation using Multiple IEA Methods
Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling.
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OsEIL2 is one of only two rice EIL genes (with OsEIL1) found to be wound-inducible; OsEIL2 is also induced by jasmonic acid.
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Recombinant OsEIL2 binds specific DNA sequences recognized by a wound-inducible tobacco EIL (EMSA), and wound-induced DNA-binding activity increased in rice nuclear extracts; suppression of OsEIL1/2 down-regulated several wound-inducible candidate target genes, indicating involvement in wound signaling.
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EIN3 is described as an essential transcription factor for ethylene signaling; OsEIL proteins are its rice EIN3-LIKE homologs.
MAOHUZI6/ETHYLENE INSENSITIVE3-LIKE1 and ETHYLENE INSENSITIVE3-LIKE2 Regulate Ethylene Response of Roots and Coleoptiles and Negatively Affect Salt Tolerance in Rice.
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OsEIL1 (MHZ6) is a rice homolog of Arabidopsis EIN3, the master transcriptional regulator of ethylene signaling; OsEIL2 is its close relative.
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Silencing OsEIL2 causes ethylene insensitivity mainly in coleoptiles of etiolated seedlings; OsEIL2 is required for ethylene-promoted coleoptile elongation.
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OsEIL2 has transactivation activity in protoplasts and localizes to the nucleus.
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OsEIL1/OsEIL2 negatively regulate salt tolerance, in part by directly activating OsHKT2;1 expression and Na+ uptake in roots; loss of function improves salt tolerance, overexpression causes salt hypersensitivity.
Deep-research report (falcon / Edison Scientific Literature) - functional annotation of rice EIL2 (Q8W3L9).
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Canonical rice ethylene signaling places OsEIL2 downstream of the receptors/OsCTR2 and OsEIN2; OsEIL1 and OsEIL2 are described as the closest functional equivalents to Arabidopsis EIN3 and as "two master regulators of rice ethylene signaling".
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OsEIL2 functions as a transcriptional activator with transactivation activity (the report maps the OsEIL2 transactivation domain to the C-terminal region, aa 344-583); it acts as a transcriptional activator of downstream genes involved in stress/senescence and coleoptile development.
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OsEIL2 fused to GFP/YFP accumulated in nuclei; nuclear fluorescence increased after ACC (ethylene precursor) and after MG132 (proteasome inhibitor), consistent with ethylene/proteasome-regulated stability.
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OsEIL2 directly binds promoters and activates transcription of target genes including OsBURP14/OsBURP16; with OsEIL1 it directly activates ROS-scavenging genes (OsVTC1-3, peroxidases) linked to coleoptile elongation and seedling emergence, and it acts as a negative regulator of salt/drought tolerance.