MTIF3 notes

Deep research

MTIF3-deep-research-claude-code.md (claude_code provider) ran for about 39 s with 2 web
searches and 6 citations (Koripella 2019, a 2020 Nat Commun paper, an eLife 2023 obesity
eQTL study, aggregator pages). It was used only for leads; claims below were checked
against cached publications.

Function

• Identification and biochemistry PMID:12095986
• 28S binding Kd about 30 nM, does not bind 55S, dissociates 55S PMID:18056078.
• C-terminal domain residues 170-175 needed for 39S displacement; C-terminal extension
  residues 247-248 needed to block fMet-tRNA binding without mRNA PMID:19239245.
• Structure on the 28S subunit PMID:30677741.
• Human knockouts: mtIF3 dispensable for leaderless mRNAs but essential for ATP6
  PMID:36629253; independent HeLa knockout PMID:32346061.
• Mouse: PMID:31903419

Judgement calls

• All 77 protein binding IPI rows (76 HuRI, 1 HI-II-14) removed; the partners are mostly
  membrane or secreted proteins.
• Ribosome disassembly (IDA) accepted: the GO definition includes subunit dissociation.
• The mitochondrion ISS row cites bovine IF-2mt (P46198) as the similarity partner, which looks
  like a with/from slip; the localisation itself is correct, so it is accepted.
• The obesity-associated MTIF3 eQTL (eLife 2023, from deep research) was not fetched or
  used; it does not bear on the molecular function.
• No Mendelian disease is established for MTIF3.