Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Expanded substrate screenings of human and Drosophila type 10 17beta-hydroxysteroid dehydrogenases (HSDs) reveal multiple specificities in bile acid and steroid hormone metabolism: characterization of multifunctional 3alpha/7alpha/7beta/17beta/20beta/21-HSD.
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The purified Drosophila type-10 17-beta-HSD (scully) has the known hydroxyacyl-CoA dehydrogenase activity plus broad hydroxysteroid dehydrogenase activities (17-beta-OH, 3-alpha-OH, 20-beta-OH, 21-OH steroids and 7-beta-OH bile acids), explained by a wide hydrophobic substrate cleft; the fly enzyme is essential in development.
"In addition to the known hydroxyacyl-CoA dehydrogenase"
Mapping organelle proteins and protein complexes in Drosophila melanogaster.
Loss of the mitochondrial protein-only ribonuclease P complex causes aberrant tRNA processing and lethality in Drosophila.
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The mitochondrial protein-only RNase P (mt:RNase P), a three-protein complex including scully (MRPP2), is responsible for 5'-end maturation of mitochondrial tRNAs; its loss causes aberrant tRNA processing and lethality in Drosophila.
"responsible for 5'-end maturation and is comprised of three"
Loss of Individual Mitochondrial Ribonuclease P Complex Proteins Differentially Affects Mitochondrial tRNA Processing In Vivo.
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Mitochondrial RNase P is a three-protein complex responsible for cleaving and processing the 5'-end of mitochondrial tRNAs; scully loss affects mt-tRNA processing in vivo.
"responsible for cleaving and processing the 5'-end of mt:tRNAs"
OpenScientist function-assignment hypothesis: scully (O18404) as the fly step-3 3-hydroxyacyl-CoA dehydrogenase
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Blinded AI-scientist analysis confirms scully is the ortholog of human HSD17B10 (type-II 3-hydroxyacyl-CoA dehydrogenase, SDR fold) with 100% conservation of the catalytic residues and confirmed (3S)-3-hydroxyacyl-CoA dehydrogenase activity, filling the fly beta-oxidation step-3 niche (Drosophila lacks a classical type-I HADH1/SCHAD ortholog).
"confirmed orthology from three independent databases"