TIM10 is a small-Tim mitochondrial intermembrane-space chaperone. Its core
activity is best represented by GO:0140309, unfolded protein holdase activity
(the current official label; “unfolded protein carrier activity” is an exact synonym):
the reconstituted Tim9-Tim10 complex “bound to the physiological substrate
ADP/ATP carrier and displayed chaperone activity in refolding the model
substrate firefly luciferase” PMID:12138093. This supports directed client
shielding and delivery, not membrane insertase activity.
The two direct GO:0140318 protein-transporter rows remain ACCEPT and are
represented as a second core function. GO:0140309 captures the substrate-state-
specific holdase behavior (maintaining hydrophobic clients without aggregation),
whereas GO:0140318 records directed delivery between TOM-side intermediates and
the TIM22 pathway. These claims overlap but emphasize experimentally supported
mechanistic dimensions rather than contradicting one another.
All 34 physical GOA rows are represented by 34 review entries. When grouped only
by term, evidence, reference, and qualifier they comprise 33 signatures; the sole
repeated signature is the pair of GO:0005515 / IPI / PMID:27107014 / enables
rows distinguished by their WITH/FROM human partners. Five generic protein-binding
rows are retained as over-annotated interaction statements, while the two intentional
cross-species human-partner rows are removed as nonphysiological yeast annotations;
the informative biology is captured by Tim9-Tim10 chaperone-complex membership,
TIM22-pathway participation, and unfolded-protein carrier activity.
All three IBA rows use PTN000113167. Current cached PAINT retains the
GO:0042721 complex-membership and GO:0045039 inner-membrane-insertion-process
IBDs. Their WITH/FROM seeds are conserved small-Tim/TIM22-pathway components,
including TIM12 (SGD:S000000295), the target TIM10
(SGD:S000003530), human TIMM10 (UniProtKB:P62072), and orthologous mouse
and worm sources for the process term. Target-in-own-WITH/FROM is valid
experimental grounding, not circularity.
GOA also carries GO:0032977 membrane insertase activity from PTN000113167 and
human TIMM10, but that assertion is absent from the current cached PAINT table.
It also conflates the small-Tim delivery role with the membrane-embedded Tim22
insertase. The row is therefore modified to GO:0140309 and recorded as stale
PAINT provenance plus role conflation. Direct work instead states that Tim10
transports carrier precursors while Tim12/Tim22 mediate insertion
[PMID:9430585, “Tim10p readily dissociated from the complex and was required
to transport carrier precursors across the outer membrane; Tim12p was firmly
bound to Tim22p and mediated the insertion of carriers into the inner
membrane.”].
Metal and zinc binding are retained as non-core. The reduced twin-CX3C motif
can bind zinc [PMID:9495346, “Both proteins contain a zinc-finger-like motif
with four cysteines and bind equimolar amounts of zinc ions.”], whereas the
mature IMS protein uses those cysteines in intramolecular disulfide bonds.
PMID:19037698 is a verified wrong identifier for the ComplexPortal IMS row:
its cached full text concerns colorectal anastomotic leakage. The biological
localization is independently established by multiple TIM10 papers, so the
annotation is accepted while the reference is explicitly marked
WRONG_IDENTIFIER; PMID:19037098 is the plausible transposed identifier.
PMID:23267104 is also a verified wrong identifier for its TIM10-TIM12 IPI row.
Full-text inspection found an E. coli-only study with no yeast, Tim10, or Tim12
mention. The physiological TIM10-TIM12 association is independently supported,
but this citation cannot support the generic interaction row.
PMID:12637749 is abstract-only. Its abstract supports the TIM22 complex as a
twin-pore translocase but does not name Tim10, Tim18, or their partner-level IPI,
so no title or non-supporting abstract sentence was used as evidence for that row.
Thirteen of the sixteen cached PMID records are abstract-only. The only cached
full texts are the unrelated PMID:19037698 paper, PMID:23267104, and
PMID:27107014. Experimental annotations were not rejected merely because assay
detail was unavailable from an abstract.
PTHR11038 PAINT for the three TIM10 IBA rows. PTN000113167GO:0042721 TIM22 mitochondrial import inner membrane insertionGO:0045039 protein insertion into mitochondrial inner membrane.PTN000113167 no longer carries GO:0032977 and now carries the sameGO:0140309 unfolded protein holdase activity term proposed as the repair forpropagation_review.source_entities to the ancestralPANTHER:PTN000113167 node rather than extant descendant donors. Target-in-own-GO:0005515 rows fromMARK_AS_OVER_ANNOTATED to REMOVE.