Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Immunocapture and MS identified the human homologues of 42 Complex I polypeptides, establishing NDUFC2/B14.5b as a subunit of human Complex I.
"we can resolve and identify the human homologues of 42 polypeptides detected so far in the more extensively studied beef heart complex I"
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Systematic knockout of each Complex I accessory subunit showed the accessory subunit set is integral to assembly and function of the human holoenzyme.
"Accessory subunits are integral for assembly and function of human mitochondrial complex I."
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM of the human respiratory megacomplex assigned individual CI subunits within the membrane arm, including NDUFC2 (PDB chain g).
"reveals the precise assignment of individual subunits of human CI and CIII"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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Review of OXPHOS assembly describing supernumerary subunits as playing essential roles in assembly, regulation and stability of the complexes.
"a large number of 'supernumerary' subunits that play essential roles in assembly, regulation and stability"
Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I.
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Characterizes MCIA-complex assembly factors that build the ND2 membrane module of Complex I, the same module in which NDUFC2 resides.
"required for building the intermediate ND2-module"
Bi-allelic pathogenic variants in NDUFC2 cause early-onset Leigh syndrome and stalled biogenesis of complex I.
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Bi-allelic NDUFC2 variants cause Leigh syndrome; complexome profiling shows loss of NDUFC2 stalls Complex I biogenesis, revealing a crucial role in assembly of the membrane arm / ND2 module.
"indicating a crucial role for NDUFC2 in the assembly of the membrane arm of complex I, particularly the ND2 module"
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Wild-type NDUFC2 cDNA rescued Complex I assembly in patient fibroblasts, confirming causality.
"Lentiviral transduction of subjects' fibroblasts with wild-type NDUFC2 cDNA increased complex I assembly"
TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I.
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TMEM242 co-purifies with the NDUFC2, ND2 and ND3 membrane subunits of Complex I, documenting a physical interaction between NDUFC2 and TMEM242.
"TMEM242-t was also bound to TMEM70 and, to a lesser extent, to subunit c and to the NDUFC2, ND2, and ND3 membrane subunits of complex I"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Reports cDNA characterization of nuclear-encoded Complex I subunits and describes the holoenzyme role in electron transport from NADH to ubiquinone coupled to proton translocation.
"Its main function is the transport of electrons from NADH to ubiquinone"
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Exocytosis of azurophil granule membrane proteins
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex