Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase.
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The vacuolar proton ATPase (V-ATPase) translocates protons into intracellular organelles or across the plasma membrane of specialised cells such as osteoclast and renal intercalated cells.
"The vacuolar proton ATPase (V-ATPase) translocates protons into intracellular organelles or across the plasma membrane of specialised cells such as osteoclast and renal intercalated cells."
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The catalytic site of the V-ATPase consists of a hexamer of three A subunits and three B subunits which bind and hydrolyse ATP and are regulated by accessory subunits C, D and E.
"are regulated by accessory subunits C, D and E. cDNAs encoding subunits C, D, and E were cloned from human osteoclastoma"
Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H(+)-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis.
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ATP6V1C1 is ubiquitously expressed; a second isoform ATP6V1C2 is expressed specifically in testes.
"Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H(+)-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis."
V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway.
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Arf6 interacts with the V0 c-subunit and ARNO with the a2-isoform of V-ATPase; the V1 C subunit is not the direct binding partner.
"Arf6 interacts with the c-subunit, and ARNO with the a2-isoform of V-ATPase"
Integral and associated lysosomal membrane proteins.
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ATP6V1C1 was detected among 17 polypeptides comprising or associated with the vacuolar adenosine triphosphatase in lysosomal membrane fractions.
"In membranes purified from placental lysosomes, we identified 58 proteins, known to reside at least partially in the lysosomal membrane. These included 17 polypeptides comprising or associated with the vacuolar adenosine triphosphatase."
Large-scale proteomics and phosphoproteomics of urinary exosomes.
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ATP6V1C1 detected in urinary exosomes by large-scale MS/MS proteomics; likely contamination from lysosomes.
"Normal human urine contains large numbers of exosomes, which are 40- to 100-nm vesicles that originate as the internal vesicles in multivesicular bodies from every renal epithelial cell type facing the urinary space."
Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.
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The lipofuscin paper does not study ATP6V1C1 directly; regulation of macroautophagy is an indirect consequence of lysosomal acidification.
"both the autophagosomes and the lysosomal system are not mandatory for the formation of lipofuscin"
Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.
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The V1 complex contains subunit C in a single copy: The V 1 ATPase is composed of three copies of subunits A, B, E, and G, and one copy of subunit C, D, F, and H.
"The V 1 ATPase is composed of three copies of subunits A, B, E, and G, and one copy of subunit C, D, F, and H"
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V-ATPase subunit C is necessary for assembly of the V1 catalytic sector.
"Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity"
Intraphagosomal pH is lowered to 5 by V-ATPase
Acidification of Tf:TfR1 containing endosome
RRAGC,D exchanges GTP for GDP
RRAGA,B exchanges GDP for GTP
v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP:SLC38A9:Arginine dissociates yielding v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP and SLC38A9:Arginine
v-ATPase:Ragulator:RagA,B:GDP:RagC,D:GDP binds SLC38A9:Arginine
v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP binds mTORC1
MITF-M-dependent ATP6V1C1 gene expression
UniProtKB entry for ATP6V1C1 (P21283)
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ATP6V1C1 subunit C is necessary for V1 assembly and has a specific function in catalytic activity.
"Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity"
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ATP6V1C1 is ubiquitously expressed.
"Ubiquitous."