Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis.
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Recombinant PIG-L has GlcNAc-PI de-N-acetylase activity in vitro, and the de-N-acetylation second step of GPI biosynthesis is conserved and essential from yeast (Gpi12p) to mammals.
"has GlcNAc-PI de-N-acetylase activity in vitro"
Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway.
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Human PIG-L is a single-pass type I ER membrane protein whose GlcNAc-PI de-N-acetylase activity is localized to the endoplasmic reticulum, retained there by a multi-component localization signal.
"We show that human PIG-L is a type I membrane protein"
Synthesis of glycosylphosphatidylinositol (GPI)
N-acetylglucosaminyl-PI + H2O -> glucosaminyl-PI + acetate
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The second GPI-synthesis reaction hydrolyzes N-acetylglucosaminyl-PI to glucosaminyl-PI and acetate in the ER membrane, catalyzed by PIG-L.
"as is the PIG-L enzyme that catalyzes it"
UniProtKB Q9Y2B2 (PIGL_HUMAN)
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PIGL catalyzes the second step of GPI biosynthesis (de-N-acetylation of GlcNAc-PI) and is an ER membrane, single-pass type I membrane protein of the PIGL family; variants cause CHIME syndrome.
"Catalyzes the second step of glycosylphosphatidylinositol"