Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Differentiation induces up-regulation of plasma membrane Ca(2+)-ATPase and concomitant increase in Ca(2+) efflux in human neuroblastoma cell line IMR-32.
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In human IMR-32 cells, induced differentiation accompanies increased PMCA2/3/4 abundance and faster calcium recovery; this is not by itself evidence that PMCA2 drives differentiation.
"during differentiation, including PMCA2, PMCA3 and PMCA4."
Alternative splicing of the first intracellular loop of plasma membrane Ca2+-ATPase isoform 2 alters its membrane targeting.
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In polarized MDCK cells, A-site splicing distinguishes apical targeting of w forms from basolateral restriction of x/z constructs. C-terminal splice state alone does not determine apical targeting.
"both PMCA2w/b and PMCA2w/a showed prominent
apical targeting"
Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region.
Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium.
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Native human corneal PMCA2 immunoreactivity has a cytoplasmic distribution in basal and wing cells; this does not establish that the integral membrane pump is a soluble cytosolic enzyme.
"PMCA2 IR was detected
cytoplasmically within basal and wing cells"
Modification of human hearing loss by plasma-membrane calcium pump PMCA2.
A functional study of plasma-membrane calcium-pump isoform 2 mutants causing digenic deafness.
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Expressed splice forms and mutants reached the CHO-cell plasma membrane at comparable levels, helping distinguish altered calcium clearance from failure of surface delivery. Some overexpressed protein was also retained in the ER.
"correctly delivered to the plasma membrane"
The interaction between endogenous calcineurin and the plasma membrane calcium-dependent ATPase is isoform specific in breast cancer cells.
Proteomic analysis of exosomes from human neural stem cells by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry.
Proteome-wide analysis of phospho-regulated PDZ domain interactions.
Cloning and expression of isoform 2 of the human plasma membrane Ca2+ ATPase. Functional properties of the enzyme and its splicing products.
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Human PMCA2 splice constructs expressed in Sf9 cells retain calcium-dependent ATPase activity and form a phosphorylated intermediate from ATP. These assays establish the general mechanism, not a particular neuronal compartment.
"Ca(2+)-dependent ATPase activity of the PMCA2 pumps"
ATP2B1-4 transport cytosolic Ca2+ to extracellular region
Ion transport by P-type ATPases
UniProtKB Q01814: ATP2B2 (human)
Plasma membrane calcium pumps in mouse olfactory sensory neurons.
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Mouse olfactory sensory neurons contain PMCA isoforms in subcellular regions including genuine cilia; the target-specific PMCA2 localization and reported knockout specificity control were checked in the separately read original paper.
"dendrite, knob, and cilia"
De novo and inherited loss-of-function variants of ATP2B2 are associated with rapidly progressive hearing impairment.
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De novo and inherited heterozygous predicted loss-of-function ATP2B2 variants are associated with progressive human hearing impairment, supporting a monogenic association beyond the earlier modifier context.
"monogenic cause of hearing impairment"
Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes.
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Human PMCA transcript abundance and alternative splicing were measured across seven tissues. This is expression context, not a direct ATP2B2-specific cardiac electrophysiology experiment.
"quantitative tissue distribution"