Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase.
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MTHFD2 has an absolute requirement for inorganic phosphate and magnesium ions to support NAD-dependent dehydrogenase activity; Pi and Mg2+ adapt an NADP-type binding site for NAD binding.
"It is unique in its absolute
requirement for inorganic phosphate and magnesium ions to support dehydrogenase
activity."
Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
A reference map of the human binary protein interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene.
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The recombinant human bifunctional enzyme is NAD-dependent and uniquely requires Mg2+ and inorganic phosphate, both of which affect NAD affinity; NADP can substitute with higher KM and lower Vmax.
"unique in its absolute
requirement for Mg2+ and inorganic phosphate."
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The enzyme is the mammalian mitochondrial homolog (yeast MIS1) proposed to provide 10-formyl-THF for formylmethionyl-tRNA synthesis in mitochondria.
"Its role is proposed to be in providing
formyltetrahydrofolate for the synthesis of formylmethionyl transfer RNA"
Metabolism of folate and pterines
MTHFD2, D2L oxidise 5,10-methylene-THF to 5,10-methenyl-THF
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MTHFD2 (with MTHFD2L) mediates the reversible oxidation of 5,10-methylene-THF to 5,10-methenyl-THF as part of mitochondrial one-carbon flux toward formate.
"dehydrogenase/cyclohydrolases D2 and D2L (MTHFD2, MTHFD2L) mediate reversible reactions"
MTHFD2, D2L oxidise 5,10-methenyl-THF to 10-formyl-THF
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MTHFD2 (with MTHFD2L) mediates the reversible conversion of 5,10-methenyl-THF to 10-formyl-THF (cyclohydrolase step).
"to convert 5,10-methylene-THF, via 5,10-methenyl-THF, to 10-formyl-THF."