Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Control of AlgU, a member of the sigma E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis.
-
MucA binds AlgU and inhibits its activity, acting as an anti-sigma factor.
"MucA was shown to act as an anti-sigma factor by binding to AlgU and inhibiting its activity."
-
Mutations in mucA cause conversion to mucoidy in cystic fibrosis isolates.
"Mutations that cause conversion to mucoidy in cystic fibrosis isolates occur frequently in mucA"
Different mutations in mucA gene of Pseudomonas aeruginosa mucoid strains in cystic fibrosis patients and their effect on algU gene expression.
-
mucA mutations in mucoid cystic fibrosis isolates, several truncating, were related to algU expression.
"In all mutated strains, algU gene expression was analyzed to determine if mutations in mucA, resulting in a strong loss of its protein, could significantly influence its function"
Use of cell wall stress to characterize sigma 22 (AlgT/U) activation by regulated proteolysis and its regulon in Pseudomonas aeruginosa.
-
MucA sequesters sigma-22 from its target promoters.
"MucA sequesters extracytoplasmic function (ECF) sigma(22) (algT/U encoded) from target promoters including PalgD for alginate biosynthesis."
-
Cell wall stress triggers rapid degradation of MucA and activation of sigma-22.
"When strain PAO1 was exposed to d-cycloserine, MucA was degraded within just 10 min, and sigma(22) was activated."
Control of Pseudomonas aeruginosa AlgW protease cleavage of MucA by peptide signals and MucB.
-
MucA is a transmembrane protein that inhibits AlgU; MucB binding blocks its cleavage by AlgW.
"The alginate response is regulated by proteolytic cleavage of MucA, a transmembrane protein that inhibits the transcription factor AlgU, and by the periplasmic protein MucB."