Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
RBMY, a probable human spermatogenesis factor, and other hnRNP G proteins interact with Tra2beta and affect splicing.
hnRNP-G promotes exon 7 inclusion of survival motor neuron (SMN) via direct interaction with Htra2-beta1.
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TRA2B binds the AG-rich SMN2 exon 7 enhancer and cooperates with hnRNP-G/RBM proteins to promote inclusion.
"hnRNP-G and RBM non-specifically bind RNA, but directly and specifically bind Htra2-beta1, an SR-like splicing factor which we have previously shown to stimulate inclusion of exon 7 through a direct interaction with the AG-rich ESE in SMN2 exon 7 pre-mRNA."
HnRNP G and Tra2beta: opposite effects on splicing matched by antagonism in RNA binding.
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TRA2B can activate or repress exon incorporation according to target context.
"We show here that hnRNP G and hTra2beta have opposite effects upon the incorporation of several exons, both being able to act as either an activator or a repressor."
Tau exon 10, whose missplicing causes frontotemporal dementia, is regulated by an intricate interplay of cis elements and trans factors.
Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization.
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CFIm68/CPSF6 interacts with TRA2B through its C-terminal alternating-charge domain.
"CF I(m)68 interacts with SRp20, 9G8, and hTra2beta, members of the SR family of splicing factors, via its C-terminal alternating charge domain."
Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins.
Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.
The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing.
Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA.
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hnRNP G-family proteins are supraspliceosomal regulators of alternative splice-site choice.
"hnRNP G is part of the supraspliceosome, where it regulates alternative splice site selection in a concentration-dependent manner."
Proteomic characterization of the human sperm nucleus.
Toward an understanding of the protein interaction network of the human liver.
Dynamic protein-protein interaction wiring of the human spliceosome.
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
The protein interaction landscape of the human CMGC kinase group.
Regulation of RAGE splicing by hnRNP A1 and Tra2β-1 and its potential role in AD pathogenesis.
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TRA2B and hnRNP A1 antagonistically regulate alternative AGER/RAGE isoform splicing.
"hnRNP A1 and Transformer2β-1 (Tra2β-1) were involved in the alternative splicing of mRAGE and esRAGE. Functionally, two factors had an antagonistic effect"
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Glucose deprivation reduces TRA2B abundance and changes the AGER/RAGE isoform ratio.
"Glucose deprivation induced an increased ratio of mRAGE/esRAGE via up-regulation of hnRNP A1 and down-regulation of Tra2β-1."
A reference map of the human binary protein interactome.
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HuRI is a proteome-scale map of human binary protein interactions.
"Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'."
Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
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Human TRA2 proteins bind GAA-repeat enhancers and activate enhancer-dependent rather than constitutive splicing.
"Neither Tra2 protein functioned in constitutive splicing in vitro, but both activated enhancer-dependent splicing in a sequence-specific manner and restored it after inhibition with competitor RNA."
The SRm160/300 splicing coactivator is required for exon-enhancer function.
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ESE-bound TRA2 proteins are coupled to U1/U2 snRNP machinery through the SRm160/300 coactivator.
"The results suggest a model for ESE function in which the SRm160/300 splicing coactivator promotes critical interactions between ESE-bound "activators" and the snRNP machinery of the spliceosome."
Mutations in tau gene exon 10 associated with FTDP-17 alter the activity of an exonic splicing enhancer to interact with Tra2 beta.
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TRA2B directly binds the MAPT exon 10 enhancer and its binding tracks enhancer-dependent splicing.
"The interaction between Tra2 beta and the exonic splicing enhancer correlates with the activity of this enhancer element in stimulating splicing."
Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA.
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TRA2B binds four enhancers in its poison exon and promotes nonproductive exon inclusion in a negative-feedback loop.
"TRA2-BETA1 binds to four enhancers present in exon 2, which activates its inclusion. Inclusion of exon 2 generates mRNAs that are not translated into proteins."
Arginine/serine-rich protein interaction domain-dependent modulation of a tau exon 10 splicing enhancer: altered interactions and mechanisms for functionally antagonistic FTDP-17 mutations Delta280K AND N279K.
Deficiency of the splicing factor Sfrs10 results in early embryonic lethality in mice and has no impact on full-length SMN/Smn splicing.
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Mouse Sfrs10/Tra2b loss did not alter full-length SMN2 splicing, demonstrating target and redundancy context.
"Surprisingly, deletion of Sfrs10 by recombinant Cre showed no impact on SMN2 splicing but increased SMN levels."
Structural basis for the dual RNA-recognition modes of human Tra2-β RRM.
Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-β1.
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The TRA2B RRM specifically recognizes an AGAA motif, with RNA contacts validated in an SMN2 splicing assay.
"The central 5'-AGAA-3' motif is specifically recognized by residues from the β-sheet of the RRM and by residues from both extremities flanking the RRM."
Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons.
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Endogenous TRA2A and TRA2B target exons show paralog compensation, with strong shifts after joint depletion.
"Here we find simultaneous--but not individual--depletion of Tra2α and Tra2β induces substantial shifts in splicing of endogenous Tra2β target exons, and that both constitutive and alternative target exons are under dual Tra2α-Tra2β control."
Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing.
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TRA2B is nuclear, colocalizes with SC35-positive speckles, and interacts with SR proteins.
"htra2-beta1 is a nuclear protein that colocalizes with SC35 in a speckled pattern."
Formation of the Spliceosomal E complex
Formation of the Spliceosomal A complex
Formation of the Spliceosomal B* complex
Formation of the Spliceosomal Pre-B complex
Formation of the Spliceosomal C* complex
Formation of the Spliceosomal B complex
Formation of the Spliceosomal Bact complex
Formation of the Spliceosomal P complex and exon ligation
Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new)
Formation of the Spliceosomal C complex containing intron lariat
Core CPA complex binds capped pre-mRNA
Recruitment of other CPA components to pre-mRNA-bound CPA core
PABPN1-stimulated PAPOL synthesizes polyA tail at distal 3'UTR PAS
PP1 dephosphorylates RNA Pol II
RBBP6 activates CPSF complex
CPSF3 cleaves pre-mRNA at distal 3'UTR PAS
U1 snRNP suppresses CPSF3-mediated pre-mRNA cleavage at intronic PAS
CPSF3 cleaves pre-mRNA at proximal 3'UTR PAS
PABPN1-stimulated PAPOL synthesizes polyA tail at proximal 3'UTR PAS
PABPN1-stimulated PAPOL synthesizes polyA tail at intronic PAS
CPA complex components dissociate from polyadenylated pre-mRNA (distal 3'UTR PAS)
CPA complex components dissociate from polyadenylated pre-mRNA (intronic PAS)
CPA complex components dissociate from polyadenylated pre-mRNA (proximal 3'UTR PAS)