GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:human/LRP3/LRP3-uniprot.txt

UniProtKB entry O75074 (LRP3_HUMAN), low-density lipoprotein receptor-related protein 3

UniProt predicts two CUB domains and four LDL-receptor class-A domains in the extracellular region, ordered CUB1–LDLRA1–LDLRA2–CUB2–LDLRA3–LDLRA4.

"FT DOMAIN 43..159 FT /note="CUB 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00059" FT DOMAIN 165..201 FT /note="LDL-receptor class A 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124" FT DOMAIN 211..250 FT /note="LDL-receptor class A 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124" FT DOMAIN 254..365 FT /note="CUB 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00059" FT DOMAIN 415..453 FT /note="LDL-receptor class A 3" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124" FT DOMAIN 454..490 FT /note="LDL-receptor class A 4" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124""

file:human/LRP10/LRP10-uniprot.txt

UniProtKB entry Q7Z4F1 (LRP10_HUMAN), low-density lipoprotein receptor-related protein 10

LRP10 is a compact LDLR-family comparator with two predicted CUB domains and four predicted LDL-receptor class-A domains.

"FT DOMAIN 28..136 FT /note="CUB 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00059" FT DOMAIN 139..175 FT /note="LDL-receptor class A 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124" FT DOMAIN 192..305 FT /note="CUB 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00059" FT DOMAIN 307..354 FT /note="LDL-receptor class A 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124" FT DOMAIN 355..397 FT /note="LDL-receptor class A 3" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124" FT DOMAIN 398..434 FT /note="LDL-receptor class A 4" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00124""
UniProt predicts an internalization role for LRP10, a paralog-specific claim that does not establish the more tentative LRP3 mechanism.

"CC -!- FUNCTION: Probable receptor, which is involved in the internalization CC of lipophilic molecules and/or signal transduction. May be involved in CC the uptake of lipoprotein APOE in liver (By similarity). {ECO:0000250}."

PMID:28340487

MicroRNA-4739 regulates osteogenic and adipocytic differentiation of immortalized human bone marrow stromal cells via targeting LRP3.

LRP3 expression was higher in the more osteogenic immortalized human bone-marrow stromal-cell clone and increased during its osteogenic induction.

"Gene expression profiling revealed significantly higher LRP3 levels in the highly osteogenic hBMSC clone imCL1 than in the less osteogenic clone imCL2, as well as a significant upregulation of LRP3 during the osteogenic induction of the imCL1 clone. Data from functional and gene expression assays demonstrated"
LRP3 perturbation in this immortalized human stromal-cell system favored osteoblast differentiation and opposed adipocyte differentiation.

"Data from functional and gene expression assays demonstrated the role of LRP3 as a molecular switch promoting hBMSC lineage differentiation into osteoblasts and inhibiting differentiation into adipocytes."
The study identified and experimentally confirmed LRP3 mRNA as a target of miR-4739 through a site in the LRP3 3' UTR.

"The TargetScan prediction algorithm, combined with functional and biochemical assays, identified LRP3 mRNA as a novel target of miR-4739, with a single potential binding site for miR-4739 located in the LRP3 3' UTR. Regulation of LRP3 expression by miR-4739 was subsequently confirmed by qRT-PCR, western blotting, and luciferase assays."

PMID:30280653

Blood-Brain Barrier: From Physiology to Disease and Back.

PMID:9693042

cDNA cloning of a new low-density lipoprotein receptor-related protein and mapping of its gene (LRP3) to chromosome bands 19q12-q13. 2.

Human and rat LRP3 cDNAs encode a 770-aa type-I membrane protein with a signal sequence, ligand-binding repeats, one transmembrane region, and a cytoplasmic tyrosine-based internalization signal.

"Human and rat LRP3 cDNAs encode a 770-amino-acid type 1 membrane protein with the following regions: a putative signal sequence, two isoleucine/leucine/valine-rich regions with an RGD sequence, two ligand-binding repeat regions, a putative transmembrane region, and a proline-rich cytoplasmic region with a tyrosine-based internalization signal."
Human LRP3 transcript was broadly detected, with the highest reported expression in skeletal muscle and ovary.

"LRP3 transcript was detected in a wide range of human tissues, with the highest expression in skeletal muscle and ovary."
Transfected CHO cells did not bind either beta-migrating VLDL or receptor-associated protein under the reported conditions.

"Despite the presence of the ligand-binding repeats, CHO cells transfected with LRP3 did not bind beta-migrating very-low-density lipoprotein or receptor-associated protein."