Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
The branched-chain amino acid transaminase gene family in Arabidopsis encodes plastid and mitochondrial proteins.
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BCAT3-GFP is imported into chloroplasts; BCAT3 complements the yeast bat1/bat2 BCAA auxotrophy.
"Restored growth on minimal medium lacking the three branched-chain amino acids confirms the respective enzymatic activities for AtBCAT-1, -2, -3, -5, and -6"
Arabidopsis branched-chain aminotransferase 3 functions in both amino acid and glucosinolate biosynthesis.
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Recombinant BCAT3 prefers the 2-oxo acids of Leu, Ile and Val but also converts MTOB, MTOP and MTOH.
"In vitro activity tests with the recombinant protein identified highest activities with the 2-oxo acids of leucine, isoleucine, and valine, but also revealed substantial conversion of intermediates of the Met chain elongation pathway."
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bcat3-1 has reduced Val and altered aliphatic glucosinolate chain-length profile; bcat3 bcat4 double mutant has strongly reduced Met-derived glucosinolates.
"This strongly suggests that BCAT3 can act as a backup of BCAT4 by transaminating Met to MTOB and thus contributes to the overall biosynthesis of Met-derived aliphatic glucosinolates, which is probably localized in plastids."
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BCAT3 most likely catalyzes terminal transamination of the chain-elongation cycle to homomethionine and dihomomethionine.
"The changes in glucosinolate proportions suggest that BCAT3 most likely catalyzes the terminal steps in the chain elongation process leading to short-chain glucosinolates: the conversion of 5-methylthiopentyl-2-oxo and 6-methylthiohexyl-2-oxo acids to their respective Met derivatives, homomethionine and dihomo-methionine, respectively."
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.
A proteomic strategy for global analysis of plant protein complexes.