Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Identification of subtilisin, Epr and Vpr as enzymes that produce CSF, an extracellular signalling peptide of Bacillus subtilis.
-
Subtilisin processes proCSF to produce the mature CSF signaling pentapeptide.
"Purified subtilisin and Vpr were shown to be capable of processing proCSF as well as at least one other Phr peptide produced by B"
-
Subtilisin is one of three sigma-H-regulated secreted serine proteases involved in Phr peptide processing.
"Mutants lacking the three proteases that fit these criteria, subtilisin, Epr and Vpr, had a defect in CSF production"
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0-A resolution.
Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation.
-
Subtilisin is not required for sporulation.
"Physiological characterization of the delta apr-684 mutation revealed no discernable effect on the formation of heat-resistant endospores, but strains carrying the mutation produced only 10% of wild-type serine protease activity"
Determination of the signal peptidase cleavage site in the preprosubtilisin of Bacillus subtilis.
Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process.
Deep research on aprE (Subtilisin E)