Gene Ontology annotation through association of InterPro records with GO terms
Regulation of polyhydroxyalkanoate biosynthesis in Pseudomonas putida and Pseudomonas aeruginosa.
Monitoring differences in gene expression levels and polyhydroxyalkanoate (PHA) production in Pseudomonas putida KT2440 grown on different carbon sources.
Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin.
Transcriptome Changes in Pseudomonas putida KT2440 during Medium-Chain-Length Polyhydroxyalkanoate Synthesis Induced by Nitrogen Limitation.
Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U.
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Pseudomonas class II PhaC polymerases are granule-associated enzymes.
"These granule-associated proteins include PHA polymerases (PhaC), PHA depolymerase (PhaZ) [9-11], phasins (PhaF and PhaI) [7,12,13] and acyl-CoA synthetase [14]."
Comprehensive proteomics analysis of polyhydroxyalkanoate (PHA) biology in Pseudomonas putida KT2440 - the outer membrane lipoprotein OprL is a newly identified phasin.
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KT2440 PhaC proteins are among the proteins associated with PHA granules.
"although PhaI, PhaC, and PhaZ are all located on the PHA granule and involved in PHA metabolism"
Deep research on phaC-II / phaC2 function in Pseudomonas putida KT2440
Falcon deep research on phaC-II / phaC2 (Q88D23, PP_5005) in Pseudomonas putida KT2440
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"PhaC2 is a **granule-associated polymerase** that catalyzes intracellular polymerization of **(R)-3-hydroxyacyl-CoA** monomers into **medium-chain-length polyhydroxyalkanoates (mcl-PHAs)**, typically containing **C6–C14** monomers depending on substrate."
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"The retrieved KT2440 literature consistently places PhaC2 in the **class II PhaC** family responsible for **mcl-PHA** production in pseudomonads, which is consistent with UniProt’s functional description “poly(3-hydroxyalkanoate) polymerase”"
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"one containing **phaC1 (PP_5003)**, **phaZ (PP_5004)**, **phaC2 (PP_5005)**, and **phaD (PP_5006)**; and an adjacent, oppositely oriented operon encoding phasins **phaF (PP_5007)** and **phaI (PP_5008)**."
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"PhaC proteins in *P. putida* are described as **granule-associated proteins (GAPs)** that coat cytoplasmic PHA storage granules along with other GAPs (e.g., depolymerase and phasins). This supports functional localization of PhaC2 at the **surface of intracellular PHA granules**"
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"PhaC2 functions in the **PHA cycle**, a dynamic synthesis/degradation system linked to central carbon metabolism and metabolic robustness. PHA accumulation is associated with **carbon excess and nutrient limitation**"
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"No retrieved text snippet provided **PhaC2-only** kinetic constants, purified-enzyme substrate preference measurements, or **single-gene phaC2 deletion/complementation phenotypes** in KT2440."
OpenScientist deep research on PSEPK PhaC2
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Supports a class II mcl-PHA synthase assignment but finds no direct enzymology or resolved division of labor for native KT2440 PhaC2.
"No direct enzymology on Q88D23 itself."
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PhaC1 and PhaC2 specialization remains unresolved in KT2440.
"PhaC1 vs. PhaC2 division of labor is unresolved."