Gene Research for GO Annotation Review

Target

• Gene symbol: DREB2A
• Organism: Arabidopsis thaliana

UniProt Context

=== UNIPROT METADATA ===
UniProt ID: O82132
Entry Name: DRE2A_ARATH
Gene Name: DREB2A
Locus Tag: At5g05410 {ECO:0000312|Araport:AT5G05410}
Gene Synonyms: ERF045 {ECO:0000303|PubMed:16407444}
Protein Name: Dehydration-responsive element-binding protein 2A
Organism: Arabidopsis thaliana (Mouse-ear cress)
NCBI Taxonomy ID: 3702
Function: Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3' (PubMed:11798174). Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription (PubMed:11798174). Promotes the expression of heat stress-inducible genes by contributing to the formation of a heat stress-specific transcriptional complex with NF-Y subunits (e.g. DPB3-1, NF-YA2 and NF- YB3) at the promoter of target genes, thus promoting heat tolerance (PubMed:25490919).
Subcellular Location: Nucleus
Subunit: Interacts with MED25 (PubMed:18552202, PubMed:21536906). Binds to DPB3-1 in the nucleus during heat-stress (PubMed:25490919).
======================

Research Objective

Prepare a comprehensive literature-backed research report for GO annotation review of the target gene.

Focus on:

1. The core biochemical activity of the gene product and any substrate specificity.
2. The activation or maturation mechanism of the protein, including proteolytic processing if relevant.
3. The best-supported subcellular localization and complexes where the active protein functions.
4. The strongest experimentally supported biological processes in the target organism, or in very close orthologs when transfer is justified.
5. Which annotations appear to reflect core function versus context-specific, pleiotropic, or over-extended roles.
6. Any evidence bearing on apoptosis, developmental cell death, neuronal roles, inflammatory signaling, pyroptosis, synaptic remodeling, protein processing, and localization to cytoplasm, cytosol, nucleus, or signaling complexes.

Output Requirements

Structure the report as:

1. Executive summary
2. Molecular function
3. Biological process roles
4. Cellular localization and complexes
5. Annotation-risk assessment
6. Key literature

Use citations for all substantive claims.

Gene Research Report for GO Annotation Review: DREB2A (Arabidopsis thaliana; At5g05410; UniProt O82132)

1. Executive summary

DREB2A (Dehydration-responsive element-binding protein 2A) is an AP2/ERF-family, DREB-subgroup transcription factor whose core biochemical activity is sequence-specific DNA binding to dehydration-responsive element/C-repeat (DRE/CRT) cis-elements with an A/GCCGAC core (often described as the DRE consensus TACCGACAT) and RNA polymerase II–dependent transcriptional activation of abiotic-stress response genes in Arabidopsis. The strongest experimental support includes (i) DRE-based binding/selection history (yeast one-hybrid isolation), (ii) reporter transactivation assays (DRE-driven and HsfA3 promoter reporters), and (iii) in planta genetic/transgenic evidence demonstrating that post-translationally derepressed DREB2A (NRD-deleted “DREB2A-CA”) drives stress-inducible gene expression and stress tolerance (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 1-2, morimoto2013stabilizationofarabidopsis pages 1-2, morimoto2013stabilizationofarabidopsis pages 4-5).

DREB2A’s activity is primarily regulated by post-translational control of protein stability and activation through a ~30-aa serine/threonine-rich negative regulatory domain (NRD). Two major E3-ligase systems negatively regulate DREB2A abundance: DRIP1/DRIP2 (RING E3 ligases) and BPM–CUL3 (CRL3BPM) adaptors, both promoting 26S proteasome-dependent turnover; genetic disruption stabilizes DREB2A and alters drought/heat phenotypes (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9, morimoto2017bpmcul3e3ligase pages 7-8). Recent authoritative reviews (2024) integrate additional regulatory inputs, including heat-induced SUMOylation near/within the NRD that inhibits BPM interaction and stabilizes DREB2A, and upstream transcriptional induction by GRF7 (repression) and HSFA1/MBF1C (heat activation) (kim2024regulatorynetworksin pages 6-7, sato2024complexplantresponses pages 8-8).

Subcellularly, DREB2A functions in the nucleus, where nuclear import is required both for transcriptional output and for proteasome-dependent turnover; two redundant NLS motifs control nuclear localization (morimoto2013stabilizationofarabidopsis pages 4-5). Under heat stress, DREB2A participates in a heat-stress-specific transcriptional complex with DPB3-1/NF-YC10 and NF-Y subunits that enhances activation of heat-inducible targets such as HsfA3 (sato2014arabidopsisdpb31a pages 10-11, sato2014arabidopsisdpb31a media d2deeb56).

No evidence in the retrieved literature supports annotating DREB2A to animal-centric pathways (apoptosis, neuronal/synaptic processes, inflammatory signaling, pyroptosis). Mentions of “cell death” largely relate to plant-specific regulators (e.g., RCD1) and should not be transferred to animal apoptosis terms without direct evidence (morimoto2013stabilizationofarabidopsis pages 11-11, sato2024complexplantresponses pages 9-10).

2. Molecular function (GO Molecular Function)

2.1 Core biochemical activity and substrate specificity

Activity: DREB2A is a sequence-specific DNA-binding transcription factor.

DNA motif specificity: DREB2A recognizes DRE/CRT elements sharing an A/GCCGAC core; DRE is often described as TACCGACAT, and DREB2A is repeatedly characterized as binding DRE/CRT to mediate drought/heat inducible transcription (sato2014arabidopsisdpb31a pages 1-2, qin2008arabidopsisdreb2ainteractingproteinsfunction pages 1-2). This is the most appropriate specificity statement for GO MF curation based on the retrieved evidence.

Transcriptional activation: In Arabidopsis protoplast assays, GFP-DREB2A activates a DRE-driven reporter (36DRE-GUS). A constitutively active NRD-deleted form (GFP-DREB2A-CA) shows ~2-fold higher transactivation than full-length GFP-DREB2A, supporting activator function (morimoto2013stabilizationofarabidopsis pages 4-5). Additionally, DREB2A cooperates with NF-Y subunits to activate the HsfA3 promoter reporter (sato2014arabidopsisdpb31a pages 10-11).

2.2 Activation/maturation mechanism

DREB2A does not require proteolytic processing for activation; instead, its activity is gated by post-translational control centered on a negative regulatory domain (NRD). Stabilization/accumulation alone can be necessary but is not sufficient to induce targets, implying additional activation steps beyond abundance control (morimoto2013stabilizationofarabidopsis pages 1-2).

Key mechanistic control points supported by primary evidence and consolidated by recent reviews:

• DRIP1/DRIP2 pathway: DRIP1/DRIP2 are DREB2A-interacting RING E3 ligases that mediate DREB2A ubiquitination and proteasome-dependent degradation and negatively regulate drought-responsive gene expression (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 1-2, qin2008arabidopsisdreb2ainteractingproteinsfunction pages 11-12).
• BPM–CUL3 pathway: BPM proteins act as substrate adaptors in a CRL3 E3 ligase complex; BPM knockdown causes DREB2A hyperaccumulation and increased thermotolerance, demonstrating NRD-mediated degradation via BPM–CUL3 (morimoto2017bpmcul3e3ligase pages 7-8).
• Stress-dependent stabilization and SUMOylation (2024 synthesis): DREB2A NRD is described as serine/threonine-rich and phosphorylated under non-stress, promoting degradation via DRIP/BPM; under heat/dehydration, these inhibitory modifications are reduced, and heat-induced SUMOylation near/within the NRD inhibits DREB2A–BPM interaction, stabilizing DREB2A (kim2024regulatorynetworksin pages 6-7, sato2024complexplantresponses pages 9-10).

2.3 Cofactor interactions relevant to MF (expert analysis)

• NF-Y/DPB3-1 synergy: DREB2A’s activator output on heat-inducible promoters can be enhanced by a NF-YA2/NF-YB3/DPB3-1 trimer, consistent with a cooperative transcriptional activation mechanism at promoters containing DRE and CCAAT elements (sato2014arabidopsisdpb31a pages 10-11, sato2014arabidopsisdpb31a media d2deeb56).
• Mediator (MED25) interaction—context-dependent: DREB2A binds MED25’s ACID domain with direct biophysical evidence (NMR/ITC/SPR/GST pull-down) using DREB2A fragments (168–335), showing conformational changes consistent with co-regulator interactions (aguilar2014interactionstudiesof pages 6-7, aguilar2014interactionstudiesof pages 1-2). However, genetic evidence suggests MED25 can act oppositely to DREB2A in drought gene induction (see Annotation risk assessment), implying MED25-related terms may require careful context selection (chong2020mediatorcomplexa pages 5-7, elfving2013functionalstudiesof pages 36-39).

3. Biological process roles (GO Biological Process)

3.1 Drought/dehydration response (strongest quantitative evidence)

A primary, experimentally supported role of DREB2A is the regulation of dehydration/drought-induced gene expression. DRIP1/DRIP2 double mutants (which stabilize DREB2A) show large transcriptome changes and improved drought-associated phenotypes:

• Transcriptome statistics: In drip1 drip2, 317 genes were >2-fold upregulated under nonstressed conditions; after 2 h dehydration, 369 genes were >2-fold upregulated. DRE-containing genes were enriched among upregulated genes (49 under nonstress; 134 under dehydration), supporting DREB2A-linked DRE regulation (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9).
• Named induced targets: RD29B and multiple dehydration-inducible genes (including GolS1 and GolS2) show elevated expression in drip1 drip2 under dehydration (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9).
• Physiological outcomes: After dehydration, ion leakage was >64% in wild type versus <32% in drip1 drip2 after 3 h; drought survival was 37.5% (WT) vs 65.4% (drip1 drip2) (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9).

These results support BP annotations centered on regulation of transcription in response to water deprivation / drought and ABA-independent osmotic stress response, with DRIP1/2 functioning as negative regulators of the DREB2A arm of the response (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9).

3.2 Heat stress response and thermotolerance

DREB2A is also strongly supported as a regulator of heat-responsive gene expression and thermotolerance:

• BPM–CUL3 control of thermotolerance: BPM knockdown (amiBPM) causes DREB2A hyperaccumulation and upregulation of DREB2A-dependent heat-inducible genes (including HsfA3 and SRC2), and increases thermotolerance in heat shock assays; epistasis indicates the enhanced heat response is DREB2A-dependent (morimoto2017bpmcul3e3ligase pages 6-7).
• Heat-stress-specific DREB2A–NF-Y complex: DPB3-1/NF-YC10 interacts with DREB2A and forms a complex with NF-Y subunits under heat stress; this complex enhances transcription from heat-inducible promoters and is summarized in a mechanistic model (sato2014arabidopsisdpb31a pages 10-11, sato2014arabidopsisdpb31a media d2deeb56).

This supports BP annotations such as response to heat and positive regulation of thermotolerance, with the caveat that promoter-level cooperative complexes contribute to target selectivity (sato2014arabidopsisdpb31a pages 10-11, sato2014arabidopsisdpb31a media d2deeb56).

3.3 High salinity / osmotic stress

Multiple sources place DREB2A within an ABA-independent transcriptional pathway induced by drought and high salinity and acting through DRE/CRT promoter elements (shinozaki2022functionalgenomicsin pages 4-6, nakashima2025transcriptionalgenenetwork pages 5-6). However, within the extracted primary experimental evidence set here, quantitative salt phenotypes specific to DREB2A are less developed than drought/heat (nakashima2025transcriptionalgenenetwork pages 5-6). For GO review, salt-related BP terms should be supported with direct experimental evidence (ideally salt-stress phenotyping or salt-induced target induction assays) if required.

4. Cellular localization and complexes (GO Cellular Component)

4.1 Subcellular localization

DREB2A functions primarily in the nucleus:

• Two redundant NLSs control nuclear localization; a D1/2 NLS double mutant becomes strongly cytosolic and has reduced transcriptional activation of a DRE reporter, linking nuclear localization to function (morimoto2013stabilizationofarabidopsis pages 4-5).
• Nuclear import is required for proteasome-dependent degradation of DREB2A, indicating that regulation of turnover occurs in the nuclear compartment (morimoto2013stabilizationofarabidopsis pages 4-5).

These observations support GO CC annotation to nucleus and argue against default annotation to cytosol except as a transient localization state for NLS mutants or specific experimental constructs (morimoto2013stabilizationofarabidopsis pages 4-5).

4.2 Protein complexes

Heat-stress DREB2A–NF-Y transcriptional complex: DPB3-1/NF-YC10 interacts with DREB2A and contributes to a trimeric NF-Y complex (NF-YA2/NF-YB3/DPB3-1) that associates with DREB2A on target promoters during heat stress. Evidence includes Y2H, in vitro pull-down, BiFC (root tissues after 37°C), co-IP, and protoplast transactivation synergy on the HsfA3 promoter (sato2014arabidopsisdpb31a pages 2-3, sato2014arabidopsisdpb31a pages 10-11). The mechanistic model is shown in the figure panel retrieved from Sato et al. 2014 (sato2014arabidopsisdpb31a media d2deeb56).

Mediator (MED25) interaction: DREB2A binds MED25’s ACID domain with biochemical/biophysical support (NMR, ITC, SPR, GST pull-down), and interaction mapping localizes the MED25-binding region within DREB2A (aguilar2014interactionstudiesof pages 6-7, elfving2013functionalstudiesof pages 36-39). Whether this should be annotated as “Mediator complex binding” depends on GO practice and whether the interaction is interpreted as direct binding to a Mediator subunit (supported) versus membership in a stable Mediator-containing complex (not directly demonstrated in planta in the extracted evidence) (aguilar2014interactionstudiesof pages 6-7, aguilar2014interactionstudiesof pages 1-2).

5. Annotation-risk assessment (curation guidance)

This section separates core function from context-specific or higher-risk interpretations.

5.1 Core vs context-specific annotations

• Core molecular function:
• sequence-specific DNA binding to DRE/CRT (A/GCCGAC core) (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 1-2, morimoto2013stabilizationofarabidopsis pages 1-2)

• transcriptional activator activity (Pol II-specific) supported by reporter assays and stress-induced gene activation in derepressed contexts (morimoto2013stabilizationofarabidopsis pages 4-5, sato2014arabidopsisdpb31a pages 10-11)

• Core biological processes:

• regulation of drought/dehydration-responsive transcription and drought tolerance phenotypes (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9)

• response to heat / thermotolerance (morimoto2017bpmcul3e3ligase pages 6-7, sato2014arabidopsisdpb31a pages 10-11)

• Context-specific regulators (should not be conflated with core MF):

• ubiquitin-dependent turnover (DRIP1/2; BPM–CUL3) is regulatory and condition-dependent; annotate DREB2A to “regulated by ubiquitination/proteasome” only if GO policy allows substrate-based regulation terms; otherwise capture E3 ligases’ roles (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 1-2, morimoto2017bpmcul3e3ligase pages 7-8).

5.2 MED25: activation vs repression ambiguity (risk)

MED25 directly binds DREB2A (strong biochemical evidence) (aguilar2014interactionstudiesof pages 6-7). However, genetic evidence and review synthesis suggest MED25 can act as a negative regulator/corepressor of DREB2A in drought responses (opposite drought phenotypes; elevated RD29A/RD29B induction in med25 summarized in one source) (chong2020mediatorcomplexa pages 5-7, elfving2013functionalstudiesof pages 36-39). Therefore:

• Avoid annotating DREB2A broadly to jasmonate/defense processes based solely on MED25’s pleiotropy.
• If “Mediator complex binding” is used, avoid implying constitutive coactivation; instead treat as context-dependent coregulator interaction pending direct in planta recruitment/functional dissection (chong2020mediatorcomplexa pages 5-7, aguilar2014interactionstudiesof pages 6-7).

5.3 Apoptosis, neuronal, inflammatory, pyroptosis, synaptic remodeling (do not annotate)

No retrieved evidence links DREB2A to these animal-centric processes. Mentions of “cell death” mainly involve plant-specific regulators (e.g., RCD1) and do not justify apoptosis/pyroptosis terms. Thus, annotations in these categories would be over-extended and unsupported for Arabidopsis DREB2A (morimoto2013stabilizationofarabidopsis pages 11-11, morimoto2017bpmcul3e3ligase pages 1-2).

6. Key literature (prioritized; includes URLs and publication dates)

Recent (prioritize 2023–2024) authoritative sources

1. Kim JS, Kidokoro S, Yamaguchi-Shinozaki K, Shinozaki K. Regulatory networks in plant responses to drought and cold stress. Plant Physiology (Mar 2024). https://doi.org/10.1093/plphys/kiae105 (kim2024regulatorynetworksin pages 6-7)

2. Consolidates NRD-centric post-translational control and stress-dependent stabilization mechanisms.

3. Sato H, Mizoi J, Shinozaki K, Yamaguchi‐Shinozaki K. Complex plant responses to drought and heat stress under climate change. The Plant Journal (Jan 2024). https://doi.org/10.1111/tpj.16612 (sato2024complexplantresponses pages 8-8, sato2024complexplantresponses pages 9-10)

4. Summarizes upstream promoter regulation (GRF7 repression; HSFA1/MBF1C activation) and heat-linked SUMOylation mechanisms.

5. Wang X et al. Transcriptional Regulators of Plant Adaptation to Heat Stress. Int. J. Mol. Sci. (Aug 2023). https://doi.org/10.3390/ijms241713297 (sato2014arabidopsisdpb31a pages 1-2)

6. Review context emphasizing NF-Y contributions to DREB2A activity under heat.

Primary experimental studies (high value for GO evidence)

1. Qin F et al. Arabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene Expression. The Plant Cell (Jun 2008). https://doi.org/10.1105/tpc.107.057380 (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9, qin2008arabidopsisdreb2ainteractingproteinsfunction pages 11-12)

2. DRIP1/DRIP2; transcriptome statistics; drought survival/ion leakage phenotypes.

3. Sato H et al. Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits. The Plant Cell (Dec 2014). https://doi.org/10.1105/tpc.114.132928 (sato2014arabidopsisdpb31a pages 2-3, sato2014arabidopsisdpb31a pages 10-11, sato2014arabidopsisdpb31a media d2deeb56)

4. Direct interaction/complex assays; promoter synergy; mechanistic model figure.

5. Morimoto K et al. Stabilization of Arabidopsis DREB2A Is Required but Not Sufficient for the Induction of Target Genes under Conditions of Stress. PLoS ONE (Dec 2013). https://doi.org/10.1371/journal.pone.0080457 (morimoto2013stabilizationofarabidopsis pages 4-5)

6. Nuclear localization/NLS logic; reporter assays; stabilization vs activation distinction.

7. Morimoto K et al. BPM-CUL3 E3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of DREB2A in Arabidopsis. PNAS (Sep 2017). https://doi.org/10.1073/pnas.1704189114 (morimoto2017bpmcul3e3ligase pages 6-7, morimoto2017bpmcul3e3ligase pages 7-8)

8. BPM–CUL3 control of DREB2A stability and thermotolerance phenotypes.

Mediator interaction (mechanistic structural evidence)

1. Aguilar X et al. Interaction Studies of the Human and Arabidopsis thaliana Med25-ACID Proteins with the … Plant-Specific Dreb2a Transcription Factors. PLoS ONE (May 2014). https://doi.org/10.1371/journal.pone.0098575 (aguilar2014interactionstudiesof pages 6-7, aguilar2014interactionstudiesof pages 1-2)
2. NMR/ITC/SPR/GST pull-down; conformational effects of MED25–DREB2A binding.

Evidence summary artifact

Table: This table summarizes GO-relevant evidence for Arabidopsis DREB2A across molecular function, biological process, and cellular component categories. It distinguishes core annotations from context-specific or higher-risk extensions and links each recommendation to specific assays, regulators, quantitative findings, and primary sources.

Figure evidence (model)

The heat-stress-specific DREB2A–NF-Y/DPB3-1 complex model is captured in Figure 9C of Sato et al. 2014 (sato2014arabidopsisdpb31a media d2deeb56).

References

1. (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 1-2): Feng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. arabidopsisdreb2a-interacting proteins function as ring e3 ligases and negatively regulate plant drought stress–responsive gene expression. Jun 2008. URL: https://doi.org/10.1105/tpc.107.057380, doi:10.1105/tpc.107.057380. This article has 648 citations.

2. (morimoto2013stabilizationofarabidopsis pages 1-2): Kyoko Morimoto, Junya Mizoi, Feng Qin, June-Sik Kim, Hikaru Sato, Yuriko Osakabe, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. Stabilization of arabidopsis dreb2a is required but not sufficient for the induction of target genes under conditions of stress. PLoS ONE, 8:e80457, Dec 2013. URL: https://doi.org/10.1371/journal.pone.0080457, doi:10.1371/journal.pone.0080457. This article has 87 citations and is from a peer-reviewed journal.

3. (morimoto2013stabilizationofarabidopsis pages 4-5): Kyoko Morimoto, Junya Mizoi, Feng Qin, June-Sik Kim, Hikaru Sato, Yuriko Osakabe, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. Stabilization of arabidopsis dreb2a is required but not sufficient for the induction of target genes under conditions of stress. PLoS ONE, 8:e80457, Dec 2013. URL: https://doi.org/10.1371/journal.pone.0080457, doi:10.1371/journal.pone.0080457. This article has 87 citations and is from a peer-reviewed journal.

4. (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 8-9): Feng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. arabidopsisdreb2a-interacting proteins function as ring e3 ligases and negatively regulate plant drought stress–responsive gene expression. Jun 2008. URL: https://doi.org/10.1105/tpc.107.057380, doi:10.1105/tpc.107.057380. This article has 648 citations.

5. (morimoto2017bpmcul3e3ligase pages 7-8): Kyoko Morimoto, Naohiko Ohama, Satoshi Kidokoro, Junya Mizoi, Fuminori Takahashi, Daisuke Todaka, Junro Mogami, Hikaru Sato, Feng Qin, June-Sik Kim, Yoichiro Fukao, Masayuki Fujiwara, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. Bpm-cul3 e3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of dreb2a in arabidopsis. Proceedings of the National Academy of Sciences, 114:E8528-E8536, Sep 2017. URL: https://doi.org/10.1073/pnas.1704189114, doi:10.1073/pnas.1704189114. This article has 155 citations and is from a highest quality peer-reviewed journal.

6. (kim2024regulatorynetworksin pages 6-7): June-Sik Kim, Satoshi Kidokoro, Kazuko Yamaguchi-Shinozaki, and Kazuo Shinozaki. Regulatory networks in plant responses to drought and cold stress. Plant Physiology, 195:170-189, Mar 2024. URL: https://doi.org/10.1093/plphys/kiae105, doi:10.1093/plphys/kiae105. This article has 278 citations and is from a highest quality peer-reviewed journal.

7. (sato2024complexplantresponses pages 8-8): Hikaru Sato, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi‐Shinozaki. Complex plant responses to drought and heat stress under climate change. The Plant journal : for cell and molecular biology, 117:1873-1892, Jan 2024. URL: https://doi.org/10.1111/tpj.16612, doi:10.1111/tpj.16612. This article has 473 citations.

8. (sato2014arabidopsisdpb31a pages 10-11): H. Sato, J. Mizoi, Hidenori Tanaka, Kyonosin Maruyama, Feng Qin, Yuriko Osakabe, Kyoko Morimoto, T. Ohori, Kazuya Kusakabe, Maika Nagata, K. Shinozaki, and K. Yamaguchi-Shinozaki. Arabidopsis dpb3-1, a dreb2a interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with nf-y subunits[c][w]. Plant Cell, 26:4954-4973, Dec 2014. URL: https://doi.org/10.1105/tpc.114.132928, doi:10.1105/tpc.114.132928. This article has 218 citations and is from a highest quality peer-reviewed journal.

9. (sato2014arabidopsisdpb31a media d2deeb56): H. Sato, J. Mizoi, Hidenori Tanaka, Kyonosin Maruyama, Feng Qin, Yuriko Osakabe, Kyoko Morimoto, T. Ohori, Kazuya Kusakabe, Maika Nagata, K. Shinozaki, and K. Yamaguchi-Shinozaki. Arabidopsis dpb3-1, a dreb2a interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with nf-y subunits[c][w]. Plant Cell, 26:4954-4973, Dec 2014. URL: https://doi.org/10.1105/tpc.114.132928, doi:10.1105/tpc.114.132928. This article has 218 citations and is from a highest quality peer-reviewed journal.

10. (morimoto2013stabilizationofarabidopsis pages 11-11): Kyoko Morimoto, Junya Mizoi, Feng Qin, June-Sik Kim, Hikaru Sato, Yuriko Osakabe, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. Stabilization of arabidopsis dreb2a is required but not sufficient for the induction of target genes under conditions of stress. PLoS ONE, 8:e80457, Dec 2013. URL: https://doi.org/10.1371/journal.pone.0080457, doi:10.1371/journal.pone.0080457. This article has 87 citations and is from a peer-reviewed journal.

11. (sato2024complexplantresponses pages 9-10): Hikaru Sato, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi‐Shinozaki. Complex plant responses to drought and heat stress under climate change. The Plant journal : for cell and molecular biology, 117:1873-1892, Jan 2024. URL: https://doi.org/10.1111/tpj.16612, doi:10.1111/tpj.16612. This article has 473 citations.

12. (sato2014arabidopsisdpb31a pages 1-2): H. Sato, J. Mizoi, Hidenori Tanaka, Kyonosin Maruyama, Feng Qin, Yuriko Osakabe, Kyoko Morimoto, T. Ohori, Kazuya Kusakabe, Maika Nagata, K. Shinozaki, and K. Yamaguchi-Shinozaki. Arabidopsis dpb3-1, a dreb2a interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with nf-y subunits[c][w]. Plant Cell, 26:4954-4973, Dec 2014. URL: https://doi.org/10.1105/tpc.114.132928, doi:10.1105/tpc.114.132928. This article has 218 citations and is from a highest quality peer-reviewed journal.

13. (qin2008arabidopsisdreb2ainteractingproteinsfunction pages 11-12): Feng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki. arabidopsisdreb2a-interacting proteins function as ring e3 ligases and negatively regulate plant drought stress–responsive gene expression. Jun 2008. URL: https://doi.org/10.1105/tpc.107.057380, doi:10.1105/tpc.107.057380. This article has 648 citations.

14. (aguilar2014interactionstudiesof pages 6-7): Ximena Aguilar, Jeanette Blomberg, Kristoffer Brännström, Anders Olofsson, Jürgen Schleucher, and Stefan Björklund. Interaction studies of the human and arabidopsis thaliana med25-acid proteins with the herpes simplex virus vp16- and plant-specific dreb2a transcription factors. PLoS ONE, 9:e98575, May 2014. URL: https://doi.org/10.1371/journal.pone.0098575, doi:10.1371/journal.pone.0098575. This article has 39 citations and is from a peer-reviewed journal.

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