Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
PEX11alpha is required for peroxisome proliferation in response to 4-phenylbutyrate but is dispensable for peroxisome proliferator-activated receptor alpha-mediated peroxisome proliferation.
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PEX11G identified as third mammalian PEX11 gene encoding a peroxisomal protein, with distinct expression properties from PEX11A and PEX11B
"we report the identification of a third mammalian PEX11 gene, PEX11 gamma, and show that it too encodes a peroxisomal protein"
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PEX11G overexpression does not induce peroxisome proliferation in fibroblasts and its expression is not detectable in fibroblasts
"PEX11 gamma differs from PEX11 alpha and PEX11 beta in that its overexpression does not induce peroxisome proliferation, its expression is not detectable in fibroblasts, and its expression is altered neither by classical PPAs nor by the loss of PEX11 alpha or PEX11 beta"
cDNA cloning and characterization of the third isoform of human peroxin Pex11p.
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PEX11G identified as third human PEX11 isoform with 241 amino acids and two putative transmembrane segments
"We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pgamma...Pex11pgamma was 241 amino-acid long, with two putative transmembrane segments"
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PEX11G shows 22-23% identity to PEX11A and PEX11B
"showing 22% and 23% amino-acid identity to Pex11palpha and Pex11pbeta, respectively"
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PEX11G localized to peroxisomal membrane by colocalization with PTS1 markers, with both N- and C-termini facing the cytosol
"Pex11pgamma was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pgamma-expressing Chinese hamster ovary cells. Pex11pgamma exposes both of the N- and C-terminal parts to the cytosol."
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PEX11G is not induced by clofibrate, showing constitutive expression similar to PEX11B
"PEX11gamma was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11beta but in contrast to inducible PEX11alpha."
PEX11 family members are membrane elongation factors that coordinate peroxisome proliferation and maintenance.
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PEX11G promotes membrane protrusion and elongation on peroxisomal surface
"Ectopic expression of proteins of the PEX11 family from yeast, plant or human lead to the formation of juxtaposed elongated peroxisomes (JEPs),which is evocative of an evolutionary conserved function of these proteins in membrane tubulation."
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PEX11G forms homodimers and heterodimers with PEX11A and PEX11B, and interacts with the fission factor FIS1
"We established the homo- and heterodimerization properties of the human PEX11 proteins and their interaction with the fission factor hFis1"
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JEPs are intermediates in peroxisome fission pathway with FIS1 as the limiting factor for progression
"Our results demonstrate that PEX11-induced JEPs represent intermediates in the process of peroxisome membrane proliferation and that hFis1 is the limiting factor for progression."
PEX11 proteins attract Mff and human Fis1 to coordinate peroxisomal fission.
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PEX11G membrane topology determined with two membrane-anchored domains and amphipathic region regulating elongation
"we establish that the PEX11gamma sequence includes two membrane-anchored domains, which dock an amphipathic region onto the peroxisomal membrane thereby regulating its elongation"
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PEX11G interacts with MFF and FIS1, linking membrane elongation to fission machinery recruitment
"We also demonstrate the presence of the mitochondrial fission factor Mff on peroxisomes and its interaction with PEX11 proteins...PEX11 proteins attract both Mff and human Fis1 (hFis1) to their site of action"
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PEX11G acts in coordination with other PEX11 proteins for membrane elongation and spatiotemporal control of peroxisome division
"PEX11gamma is required and acts in coordination with at least one of the other PEX11 proteins to protrude the peroxisomal membrane...the concerted interaction of PEX11 proteins provides spatiotemporal control for growth and division of peroxisomes"
Fission impossible (?) - new insights into disorders of peroxisome dynamics.
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PEX11G overexpression can partially rescue catalase import defects and abnormal peroxisome morphology in PEX11B-deficient patient fibroblasts under elevated-temperature stress
"Carmichael et al. summarize that in PEX11B-deficient patient fibroblasts, PEX11G mRNA and protein decrease substantially at elevated temperature (40 C), and that overexpression of PEX11G can partially rescue catalase import defects and abnormal peroxisome morphology under this stress condition."
A reference map of the human binary protein interactome.
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High-throughput Y2H interactome study detecting PEX11G interactions with AQP6, ERGIC3, KCNJ6, TMEM14B, and TMEM86B, which lack obvious biological relevance to peroxisome function
"HI-III-20 (Human Interactome obtained from screening Space III, published in 2020), contains 52,569 verified PPIs involving 8,275 proteins"