Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Evidence for the existence of a pristanoyl-CoA oxidase gene in man.
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Cloned the human ACOX3 cDNA (700 aa) from liver, showing 75% identity with rat pristanoyl-CoA oxidase and a C-terminal PTS1 (SKL) peroxisomal targeting signal; the gene maps to 4p15.3 and is expressed at very low level in liver.
"harbouring a peroxisomal C-terminal-targeting"
A novel case of ACOX2 deficiency leads to recognition of a third human peroxisomal acyl-CoA oxidase.
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Substrate-specificity studies of human ACOX1/2/3 showed ACOX1 oxidizes straight-chain fatty acids, ACOX2 handles bile-acid intermediates, and both ACOX2 and ACOX3 degrade branched-chain fatty acids; ACOX3 is expressed in normal human tissues at mRNA and protein level.
"both ACOX2 and ACOX3 are involved in the degradation"
Mammalian peroxisomal acyl-CoA oxidases. III. Molecular characterization of human branched chain fatty acyl-CoA oxidase.
Defining the membrane proteome of NK cells.
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High-throughput mass-spectrometry membrane-proteome survey of an NK-like cell line (YTS) identifying >1800 proteins; source of the HDA "membrane" annotation. Not specific to ACOX3 function.
"membrane proteome of NK cells"
(2S)-pristanoyl-CoA + O2 => trans-2,3-dehydropristanoyl-CoA + H2O2 (ACOX3)
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Reactome reaction modeling ACOX3 as the peroxisomal enzyme oxidizing (2S)-pristanoyl-CoA to trans-2,3-dehydropristanoyl-CoA and H2O2.
"Peroxisomal ACOX3 catalyzes the reaction of (2S)-pristanoyl-CoA and O2 to form trans-2,3-dehydropristanoyl-CoA and H2O2"
Beta-oxidation of pristanoyl-CoA
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Pathway context: pristanoyl-CoA (from alpha-oxidation of dietary phytanic acid) is degraded by three cycles of peroxisomal beta-oxidation, the first dehydrogenation of which is catalyzed by ACOX3.
"further catabolized by three cycles of peroxisomal beta-oxidation"
Cargo of PEX5S,L translocates from the cytosol to the peroxisomal matrix
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PEX5-mediated import step by which PTS1 cargo (including ACOX3) is translocated from the cytosol into the peroxisomal matrix; basis for the transient cytosol and matrix localization annotations.
"the cargo protein is released into the peroxisomal matrix"
PEX5S,L:Cargo binds PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Module)
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PEX5:cargo binding to the peroxisomal docking/translocation module; basis for a transient cytosol localization annotation of the imported cargo.
"the Docking and Translocation Module (PEX13:PEX14:PEX2:PEX10:PEX12)"