Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Fluorescent sensors of siderophores produced by bacterial pathogens.
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A labelled PfeA sensor binds ferric enterobactin with FepA-like ligand preference.
"First, the EcoFepA orthologs KpnFepA1658 (82% identity) and PaePfeA (61% identity) had the same ligand preferences as EcoFepA (FeEnt > FeEnt∗ > FeGEnt)."
Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeruginosa.
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A receptor-less mutant grows poorly on ferric enterobactin; the cloned pfeA restores the outer-membrane protein and enterobactin-dependent 55Fe uptake in E. coli fepA.
"Moreover, the fragment complemented the fepA mutation in MT912, restoring both growth in EDDHA-containing medium and enterobactin-dependent uptake of 55Fe3+."
The Pseudomonas aeruginosa pirA gene encodes a second receptor for ferrienterobactin and synthetic catecholate analogues.
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PirA is a second ferric-enterobactin receptor redundant with PfeA.
"Ferric-enterobactin also failed to stimulate growth of the double pfeA-pirA mutant although, like its synthetic analogues, it stimulated growth of the corresponding single mutants."