DNAH3 notes

Identity and structure

UniProt Q8TD57, 4116 aa; full heavy-chain architecture [file:human/DNAH3/DNAH3-uniprot.txt].
IDAc heavy chain in human respiratory axonemes PMID:37258679.

Testis and trachea PMID:9256245; in testis, "Initially, DNAH3 was expressed in the cytoplasm of spermatogonia and spermatocytes, and then it clearly transferred into the flagellum of early and late spermatids." PMID:39503742.

"Combined comparative proteomics and co-immunoprecipitation analyses demonstrated that DNAH3 forms a complex with DNALI1." and the stem domain mediates it PMID:41731497.

Patients + KO: "the absence of DNAH3 led to decreased expression of other IDA-associated proteins, including DNAH1, DNAH6 and DNALI1" PMID:39503742.
KO: absence of IDAs; DNAH6, DNAH10, DNALI1 reduced PMID:41731497.
Patients: CP loss, MS/FS dislocation, partial IDA absence; two KO lines with AT; airway and brain cilia grossly normal in KO PMID:38312775.
OAT/MMAF patients PMID:39588341.
Conflicting: KO sperm with normal morphology, energy-metabolism defect PMID:39774634.

Core MF: minus-end-directed MT motor activity; dynein light intermediate chain binding (direct DNALI1 co-IP) ACCEPTED.
Dynein intermediate chain binding UNDECIDED (IBD seeded only by Dictyostelium cytoplasmic dynein).
NAS (PMID:9256245) axonemal dynein complex -> inner dynein arm; cilium-dependent cell motility -> flagellated sperm motility.

Human Q8TD57 and mouse Q8BW94 have identical IBA sets (8 terms) in QuickGO; no family split. DNAH3 seeds the PTN008951089 IBDs (IDA assembly, sperm flagellum assembly).

Falcon deep research failed (Edison API authentication 503 errors on repeated attempts).