Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

PMID:16189514

Towards a proteome-scale map of the human protein-protein interaction network.

PMID:19946888

Defining the membrane proteome of NK cells.

PMID:22119785

Defining human ERAD networks through an integrative mapping strategy.

Identified EMC8 (as COX4NB) as a high-confidence component of the mammalian ER membrane protein complex (mEMC), establishing EMC complex membership and ER membrane/cytoplasm localization.

PMID:26496610

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

PMID:28514442

Architecture of the human interactome defines protein communities and disease networks.

PMID:29242231

The ER membrane protein complex is a transmembrane domain insertase.

The EMC is a transmembrane-domain insertase that catalyzes insertion of tail-anchored and other substrates into the ER membrane in a reconstituted system; EMC8 is a subunit of this complex.

PMID:29809151

The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins.

EMC engages ribosomes cotranslationally and is required for biogenesis of multipass membrane proteins; depletion of EMC subunits impairs insertion.

PMID:30021884

Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.

PMID:30415835

EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.

EMC mediates cotranslational insertion of the first TMD of GPCRs and other multipass proteins and cooperates with Sec61 to ensure accurate topogenesis; EMC knockout impairs correct topology.

PMID:32296183

A reference map of the human binary protein interactome.

PMID:32439656

Structural basis for membrane insertion by the human ER membrane protein complex.

Cryo-EM structure of the human nine-subunit EMC; substrate insertion occurs via an enclosed hydrophilic vestibule formed by EMC3 and EMC6, with EMC8 resolved as a cytosolic-facing subunit.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

PMID:35271311

OpenCell: Endogenous tagging for the cartography of human cellular organization.

PMID:37196677

EMC chaperone-Ca(V) structure reveals an ion channel assembly intermediate.

Cryo-EM of human EMC bound to a CaV1.2-CaVbeta3 assembly intermediate; EMC8 forms a specific cytosolic client-docking interface ("Cyto dock"/"EMC8 site", ~962 A^2 centered on the last helix of EMC8) with CaVbeta3, binding two CaVbeta loops and ordering an otherwise disordered CaVbeta region, demonstrating an EMC8-specific chaperone/assembly role beyond generic insertion.

PMID:32459176

The architecture of EMC reveals a path for membrane protein insertion.

EMC2 forms an alpha-solenoid that clamps around EMC8 in the cytosolic basket; EMC8 is a ~23 kDa monomer ~44% identical to EMC9, and EMC8 and EMC9 are mutually exclusive subunits (no ternary EMC2-EMC8-EMC9 complex forms), supporting EMC8 as a structural assembly/scaffold subunit of the cytosolic module.

PMID:35287476

The Function, Structure, and Origins of the ER Membrane Protein Complex.

Authoritative review of EMC function, structure, and evolution; places EMC2, EMC8, and EMC9 as the cytosolic subunits and EMC8/EMC9 as mutually exclusive paralogs of the cytosolic cradle that engages membrane-protein clients before insertion.

PMID:38517390

Structural insights into human EMC and its interaction with VDAC.

Cryo-EM of human EMC apo and VDAC1-bound states at mitochondria-ER contact sites, with a hydrophilic-vestibule "gating plug" conformational change proposed to regulate EMC function; EMC8 is a cytosolic subunit of the complexes studied (no EMC8-specific mechanistic role assigned in this study).

file:human/EMC8/EMC8-uniprot.txt

UniProt entry O43402 (EMC8_HUMAN), ER membrane protein complex subunit 8

EMC8 is a cytosolic, peripheral subunit (cytoplasmic side) of the ER membrane protein complex (EMC), an energy-independent TMD insertase for newly synthesized membrane proteins; mediates cotranslational multipass and post-translational tail-anchored insertion. EMC8 and EMC9 are mutually exclusive subunits; EMC8 belongs to the EMC8/EMC9 family and contains an MPN domain.