GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

InterPro2GO mapping electronically transfers KCS/FAE1 condensing-enzyme and acyltransferase GO terms onto FAE1 from its KCS/3-ketoacyl-CoA synthase InterPro signatures; consistent with the experimentally established beta-ketoacyl-CoA synthase activity.

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

ARBA machine-learning rules propagate GO:0005783 (endoplasmic reticulum) localization to FAE1 from the KCS family; consistent with and corroborated by experimental ER localization of Arabidopsis KCSs including KCS18.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined IEA pipelines (InterPro/UniRule/ARBA) converge on fatty-acid elongation and integral-membrane terms for FAE1, all consistent with KCS18 as the ER membrane-bound condensing enzyme of the seed FAE complex.

GO_REF:0000122

AtSubP analysis

AtSubP subcellular-prediction-based annotation predicts GO:0009507 (chloroplast) localization for FAE1, which contradicts the experimentally established ER/microsomal localization of KCS18 and was therefore marked REMOVE in this review.

PMID:11341960

Active-site residues of a plant membrane-bound fatty acid elongase beta-ketoacyl-CoA synthase, FAE1 KCS.

Identifies catalytic active-site residues of the FAE1 KCS condensing enzyme, confirming its beta-ketoacyl-CoA synthase activity.

PMID:12135493

Engineering and mechanistic studies of the Arabidopsis FAE1 beta-ketoacyl-CoA synthase, FAE1 KCS.

FAE1 KCS is active on saturated and monounsaturated C16-C18 acyl-CoAs, elongating to C20-C22 VLCFAs, with no activity on polyunsaturated substrates; microsome (ER) membrane localization.

PMID:36798704

Tackling functional redundancy of Arabidopsis fatty acid elongase complexes.

Systematic reconstitution of Arabidopsis KCS isoforms; KCS18 localizes to the ER and shows a strong bias toward monounsaturated C20 products.

PMID:7734965

Directed tagging of the Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene with the maize transposon activator.

Identifies FAE1 by transposon tagging; fae1 mutant seeds are strongly depleted of very-long-chain fatty acids, establishing FAE1's role in seed VLCFA elongation.

file:ARATH/FAE1/FAE1-deep-research-falcon.md

Falcon/Edison deep research report for FAE1/KCS18, Arabidopsis thaliana

Synthesizes FAE1/KCS18 as the seed-specialized, chain-length-determining condensing enzyme of the ER FAE complex; fae1 reduces seed VLCFAs from ~28% to <1%; a key metabolic-engineering control point for long-chain monounsaturated seed oils.