Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
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Lysates of a PA2133-overexpressing strain degraded 71% of added c-di-GMP (Table 1, read in PMC full text).
"Analysis of the phenotypes of DGC and PDE mutants and overexpressing clones revealed that certain virulence-associated traits are controlled by multiple DGCs and PDEs through alterations in c-di-GMP levels."
A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels.
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Plasmid expression of PA2133 removes detectable c-di-GMP from a wspF mutant.
"wspF mutant cells carrying the PA2133 expression vector had undetectable levels of c-diGMP"
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Plasmid expression of PA2133 abolishes attachment of wild-type cells.
"the ability of wild-type cells to attach to surfaces was also completely abolished when PA2133 was expressed"
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A PA2133 loss-of-function mutant has no attachment phenotype.
"A PA2133 mutant resembled the wild type in attachment assays."
Induction of Native c-di-GMP Phosphodiesterases Leads to Dispersal of Pseudomonas aeruginosa Biofilms.
Annotation inferences using phylogenetic trees