Official human identity: HGNC:29514, UniProtKB:Q6ZMI3. All 13 raw contexts are unsuffixed, without negation or annotation extensions.
The official 551-residue Q6ZMI3-1 and 427-residue Q6ZMI3-2 sequences are distinct natural splice products. The shorter sequence exactly matches canonical residues 125–551; it is not a label for the proteolytically released ectodomain. No unique activity is assigned to it here. Historical CAD98018 sequence conflict V376A is retained as source context, not interpreted as a modern canonical variant.
PAINT family PTHR23192 was inspected through its actual tree, alignment, ancestral mapping and IBD records. The target is AN489/PTN002509188. The three IBA placements and all literal donor sets were replayed independently. The cell-surface self-donor is experimentally grounded.
Primary construct boundaries are preserved: rat clone AY266116 in PMID:16039564 is 549 residues; modern human canonical sequence is 551 residues. Recombinant fragments, Fc fusions and assay hosts do not define natural human isoforms. The source-specific assay and access boundaries are recorded in reference reviews.
HPA050873 reports U2OS plasma membrane and MCF-7/SuSa vesicles. These cell-line observations are not universal localization or isoform assignments.
The official Matrisome portal links the human masterlist: sheet1 row63 identifies GLDN/HGNC29514/Q6ZMI3 as Core matrisome / ECM Glycoproteins. An older linked workbook agrees at row62. Category membership is distinct from a specific human peptide or tissue measurement.
No NEW annotations are proposed. The axon IEA decision is restricted to physiological compartment mapping and does not deny extracellular binding to axonal receptors.
The earlier no-NEW statement records the initial review and is superseded by the single clustering proposal below. See primary evidence and scope for the cited assay and localization anchors. The original 13 annotation contexts remain unchanged; one proposed ISS annotation is separate.
The broad signal-transduction IBA remains as non-core evidence, with its verified ancestral placement retained. The core now names channel clustering. Cell-line-specific HPA staining remains supported but non-core; the Matrisome assertion remains accepted while the core location uses extracellular region.
The three plasma-membrane annotations are retained as non-core. This aligns the supported precursor locations with the extracellular/cell-surface core; it does not reject membrane localization. The axon source concern is a SUSPECTED UniProt CHECK, preserving the distinct rat and mouse citation chains in the evidence record. No corrected compartment or universal mapping error is asserted.
[PMID:16039564, Figure 4D–H, "no gold particles were detected
in the axon"]. Original-paper access and the abstract-only cache remain distinguished in the source guide.
The previous validator warning was: Inconsistent review actions for term GO:0005886 (plasma membrane): ACCEPT (IEA, ISS); KEEP_AS_NON_CORE (IDA). It came from validation/validator.py (inconsistent_review_actions), separately from the action-consistency rule table. Its original output and the prior DRAFT history are preserved; status must be recomputed after applying this follow-up.