Systematic analysis and nomenclature of mammalian F-box proteins.
Array MAPPIT: high-throughput interactome analysis in mammalian cells.
A High-Density Map for Navigating the Human Polycomb Complexome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Multimodal cell maps as a foundation for structural and functional genomics.
AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex
NEDD8:AcM-UBE2M binds CRL1 E3 ubiquitin ligase complex
CAND1 binds cytosolic CRL E3 ubiquitin ligases
COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
MyrG-DCUN1D3 binds CRL1 E3 ubiquitin ligase complex
Transfer of Ub from E2 to substrate and release of E2
Release of E3 from polyubiquitinated substrate
Polyubiquitination of substrate
Interaction of E3 with substrate and E2-Ub complex
Falcon deep research report for human FBXW9
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FBXW9 binds SKP1 (array MAPPIT screen validated by co-immunoprecipitation in HEK293T) and co-purifies with CUL1 in AP-MS of SCF assemblies, providing direct experimental evidence that it is an SCF/CRL1 substrate-recognition adaptor rather than an enzyme.
"FBXW9 was identified as a SKP1-interacting prey in a high-throughput **array MAPPIT** interactome screen and validated by **co-immunoprecipitation** in **HEK293T** cells"
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FBXW9 is a direct p53 transcriptional target (ChIP/luciferase/Nutlin-3 evidence) whose ectopic expression suppresses proliferation of p53-deficient cells, positioning it downstream of p53 tumor-suppressor signaling.
"ChIP-seq/ChIP-qPCR showed p53 occupancy near Fbxw9 promoter; p53 increased luciferase reporter activity; p53 knockdown reduced expression; Nutlin-3 induced Fbxw9 in p53-WT but not p53-null human cells"
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In glioblastoma stem-like cells FBXW9 is a CREB-bound transcriptional target downstream of an IGFBP5-ROR1/HER2 axis, and FBXW9 knockdown reduces invasion, with high FBXW9 associated with worse survival.
"siRNA against FBXW9 significantly decreased invasion capacity in X01 and 448 GSC lines; high FBXW9 associated with worse glioma/LGG survival"
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In breast cancer cells FBXW9 knockdown reduces proliferation and induces G0/G1 arrest with increased p21, but TP53 as an FBXW9 ubiquitination substrate remains predicted (UbiBrowser), not biochemically validated.
"TP53 as substrate remains predicted, not biochemically validated by ubiquitination/degradation assay"
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No directly validated endogenous ubiquitination substrate for human FBXW9 has been established; the molecular substrate repertoire and degron remain unresolved.
"No directly validated endogenous ubiquitination substrate for human FBXW9 was recovered; no direct catalytic assay, no substrate degron definition"