Gene Ontology annotation through association of InterPro records with GO terms

InterPro-to-GO mapping (IPR000433 ZZ-type zinc finger) assigns zinc ion binding to JOKA2; the ZZ finger in JOKA2 is degenerate.

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

SwissProt keyword-derived (SPKW) annotations from keywords "Autophagy", "Protein transport"/"Transport" and "Cytoplasmic vesicle"; present in the snapshot but removed from the current GOA release after GOA retired the keyword2GO pipeline for cellular organisms.
For JOKA2 the "Autophagy" keyword reflects genuine biology (a bona fide NBR1/p62-family selective-autophagy cargo receptor); the keyword-derived terms are simply broader than the gene's specific macroautophagy / cargo-adaptor functions.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Subcellular-location vocabulary mapping assigns vacuole (SL-0272) and autophagosome (SL-0023) to JOKA2; both are independently supported by experimental data.

PMID:26765567

An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor.

Potato Joka2 is a selective-autophagy cargo receptor of Solanaceous plants that binds ATG8CL via its AIM motif (819-WDPI-822); the Joka2-AIM mutant loses ATG8CL binding.
Joka2 (but not Joka2-AIM) increases the number of GFP:ATG8CL autophagosomes and activates ATG8CL-mediated selective autophagy, and Joka2:RFP colocalizes with GFP:ATG8CL-labelled autophagosomes.
Overexpression of Joka2 (but not Joka2-AIM) restricts P. infestans lesion size and silencing increases disease lesions, so Joka2-mediated selective autophagy contributes to defense; the P. infestans effector PexRD54 outcompetes Joka2 for ATG8CL binding.

PMID:29932422

Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen.

Selective autophagy employs specialized autophagy cargo receptors that bind ATG8 on autophagosome membranes and recruit specific cargoes to autophagosomes; autophagosomes are carried to the vacuole for recycling.
The Joka2/NBR1-mediated selective autophagy pathway contributes to defense against P. infestans; during infection Joka2/ATG8CL-labelled autophagosomes are diverted to the perihaustorial/extrahaustorial membrane to restrict pathogen growth.
JOKA2 localizes to the vacuole (UniProt SUBCELLULAR LOCATION, ECO:0000269).

file:SOLTU/JOKA2/JOKA2-deep-research-falcon.md

Deep-research report (falcon / Edison Scientific Literature) - functional annotation of potato JOKA2 (M1BJF6), NBR1-family selective-autophagy receptor.

Synthesizes the Dagdas et al. 2016 (eLife, PMID:26765567) and 2018 (eLife, PMID:29932422) potato/Solanaceae studies with the broader plant NBR1 literature, concluding JOKA2 is best annotated as a selective-autophagy cargo receptor/adaptor that binds ATG8 (notably ATG8CL) via an AIM/LIR and (by domain/family inference) binds ubiquitinated cargo via its UBA domain.
Describes the canonical NBR1/Joka2 modular architecture (PB1 - ZZ - NBR1/FW - UBA - AIM - UBA), matching the UniProt M1BJF6 domain set; PB1 mediates oligomerization, ZZ+FW cargo recognition, UBA ubiquitin binding, and the AIM ATG8 binding.
Localizes JOKA2 to cytoplasmic ATG8CL-positive autophagosomes and, during infection, to perihaustorial autophagosomes at the extrahaustorial membrane; PexRD54 antagonises Joka2 by competing for ATG8CL.
Notes the 2024 Arabidopsis NBR1 mechanistic expansion (LLPS/condensates and recognition of non-ubiquitinated cargo via ZZ+FW), which by inference may also apply to potato JOKA2 but is not directly demonstrated for it.