Shared GAPDH family evidence (TDH1/TDH2/TDH3 are paralogous NAD+-dependent phosphorylating GAPDHs, EC 1.2.1.12):
- Activity partition among paralogs PMID:3905788; all three active PMID:3905788.
- Same relative expression on glucose and ethanol (supports gluconeogenic as well as glycolytic role) PMID:3905788.
- Tdh2/Tdh3 homotetramers; Tdh3 lower Vmax PMID:3905788.
- Cell wall + cytosol PMID:11158358; PMID:11158358.
- Side activity: NAD(P)H hydratase [UniProt:P00359 "FUNCTION: As a side activity, catalyzes the hydration of the"]; NADP binding IEA from IPR006424 judged over-annotation for an NAD-specific GAPDH.
- Peripheral mitochondrial pool PMID:16962558; minor lipid-particle co-fractionation PMID:10515935.
- Melatonin affinity pull-down hit PMID:31708896; weak affinity-capture evidence marked over-annotated.
- NO/S-nitrosation and apoptosis (TDH2/TDH3 IMP) PMID:17726063 (abstract only; kept non-core).
TDH3-specific (moonlighting, all kept non-core):
- RNA binding: crystal structure + RNA specificity PMID:25288528; RIP-chip PMID:18959479; FAS1 mRNA PMID:37792491.
- Heme buffering/allocation, TDH3-specific among paralogs PMID:27247412; PMID:27247412; conserved His needed for heme binding PMID:30012884.
- Mitochondrion in UniProt from proteomics [UniProt:P00359 "Mitochondrion {ECO:0000269|PubMed:16823961}."].
Decisions: as TDH1 (but mitochondrion IEA kept non-core, matching UniProt), plus KEEP_AS_NON_CORE RNA/mRNA binding, heme binding, heme transport, nucleus, apoptotic process, ROS metabolic process.